Anti-PPAP2B Antibody (CAB15743)
- SKU:
- CAB15743
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-PPAP2B Antibody |
| Antibody SKU: | CAB15743 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PPAP2B (NP_003704.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, LO2, MCF7, Mouse brain, Mouse kidnay, Rat lung, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PPAP2B (NP_003704.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MQNY KYDK AIVP ESKN GGSP ALNN NPRR SGSK RVLL ICLD LFCL FMAG LPFL IIET STIK PYHR GFYC NDES IKYP LKTG ETIN DAVL CAVG IVIA ILAI |
| Gene ID: | 8613 |
| Uniprot: | O14495 |
| Cellular Location: | Cell membrane, Golgi apparatus, Multi-pass membrane protein, trans-Golgi network membrane |
| Calculated MW: | 35kDa |
| Observed MW: | 38kDa |
| Synonyms: | PLPP3, Dri42, LPP3, PAP2B, PPAP2B, VCIP |
| Background: | The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is a membrane glycoprotein localized at the cell plasma membrane. It has been shown to actively hydrolyze extracellular lysophosphatidic acid and short-chain phosphatidic acid. The expression of this gene is found to be enhanced by epidermal growth factor in Hela cells. |
| UniProt Protein Function: | Function: Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA = PA > C-1-P > S-1-P. May be involved in cell adhesion and in cell-cell interactions. |
| UniProt Protein Details: | Catalytic activity: A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate. Enzyme regulation: Inhibited by sphingosine, zinc ions and propanolol. Not inhibited by N-ethylmaleimide treatment. Subunit structure: Homodimer. This complex seems not to be involved in substrate recognition, it may confer only structural or functional stability. Ref.9 Subcellular location: Golgi apparatus › trans-Golgi network membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Tissue specificity: Ubiquitously expressed. Highly expressed in heart and placenta. Induction: By EGF, VEGF, FGF2 and phorbol myristate acetate (PMA). Ref.1 Post-translational modification: N-glycosylated. Contains high-mannose oligosaccharides. Sequence similarities: Belongs to the PA-phosphatase related phosphoesterase family. Sequence caution: The sequence AAB50222.1 differs from that shown. Reason: Frameshift at position 225. |
| NCBI Summary: | The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is a membrane glycoprotein localized at the cell plasma membrane. It has been shown to actively hydrolyze extracellular lysophosphatidic acid and short-chain phosphatidic acid. The expression of this gene is found to be enhanced by epidermal growth factor in Hela cells. [provided by RefSeq, Mar 2010] |
| UniProt Code: | O14495 |
| NCBI GenInfo Identifier: | 29171740 |
| NCBI Gene ID: | 8613 |
| NCBI Accession: | NP_003704.3 |
| UniProt Secondary Accession: | O14495,Q96GW0, Q99782, B2R651, D3DQ52, |
| UniProt Related Accession: | O14495 |
| Molecular Weight: | 35,116 Da |
| NCBI Full Name: | lipid phosphate phosphohydrolase 3 |
| NCBI Synonym Full Names: | phosphatidic acid phosphatase type 2B |
| NCBI Official Symbol: | PPAP2B |
| NCBI Official Synonym Symbols: | LPP3; VCIP; Dri42; PAP2B |
| NCBI Protein Information: | lipid phosphate phosphohydrolase 3; PAP2 beta; phosphatidate phosphohydrolase type 2b; type-2 phosphatidic acid phosphatase-beta; vascular endothelial growth factor and type I collagen inducible |
| UniProt Protein Name: | Lipid phosphate phosphohydrolase 3 |
| UniProt Synonym Protein Names: | PAP2-beta; Phosphatidate phosphohydrolase type 2b; Phosphatidic acid phosphatase 2b; PAP-2b; PAP2b; Vascular endothelial growth factor and type I collagen-inducible protein |
| Protein Family: | Lipoma-preferred partner |
| UniProt Gene Name: | PPAP2B |
| UniProt Entry Name: | LPP3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PPAP2B antibody (CAB15743) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
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