Anti-POU6F1 Antibody (CAB7299)
- SKU:
- CAB7299
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-POU6F1 Antibody |
| Antibody SKU: | CAB7299 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human POU6F1 (NP_002693.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW620, 22Rv1, HepG2, Raji, Mouse kidney, Mouse liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human POU6F1 (NP_002693.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPGI SSQI LTNA QGQV IGTL PWVV NSAS VAAP APAQ SLQV QAVT PQLL LNAQ GQVI ATLA SSPL PPPV AVRK PSTP ESPA KSEV QPIQ PTPT VPQP AVVI ASPA PAAK PSAS APIP ITCS ETPT VSQL VSKP HTPS LDED GINL EEIR EFAK NFKI RRLS LGLT QTQV GQAL TATE GPAY SQSA ICRF EKLD ITPK SAQK LKPV LEKW LNEA ELRN QEGQ QNLM EFVG GEPS KKRK RRTS FTPQ AIEA LNAY FEKN PLPT GQEI TEIA KELN YDRE VVRV WFCN RRQT LKNT SKLN VFQI P |
| Gene ID: | 5463 |
| Uniprot: | Q14863 |
| Cellular Location: | Nucleus |
| Calculated MW: | 32kDa |
| Observed MW: | 45kDa |
| Synonyms: | POU6F1, BRN5, MPOU, TCFB1 |
| Background: |
| UniProt Protein Function: | POU6F1: Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3'). Belongs to the POU transcription factor family. Class- 6 subfamily. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 12q13.13 Cellular Component: nucleoplasm; nucleus; actin cytoskeleton Molecular Function:DNA binding; transcription factor activity Biological Process: muscle development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; heart development; brain development |
| UniProt Code: | Q14863 |
| NCBI GenInfo Identifier: | 2495306 |
| NCBI Gene ID: | 5463 |
| NCBI Accession: | Q14863.1 |
| UniProt Secondary Accession: | Q14863,Q15944, Q6DK47, Q7Z7P6, |
| UniProt Related Accession: | Q14863 |
| Molecular Weight: | 301 |
| NCBI Full Name: | POU domain, class 6, transcription factor 1 |
| NCBI Synonym Full Names: | POU class 6 homeobox 1 |
| NCBI Official Symbol: | POU6F1 |
| NCBI Official Synonym Symbols: | BRN5; MPOU; TCFB1 |
| NCBI Protein Information: | POU domain, class 6, transcription factor 1; brn-5; brain-5; mPOU homeobox protein; brain-specific homeobox/POU domain protein 5 |
| UniProt Protein Name: | POU domain, class 6, transcription factor 1 |
| UniProt Synonym Protein Names: | Brain-specific homeobox/POU domain protein 5; Brain-5; Brn-5; mPOU homeobox protein |
| Protein Family: | POU domain, class 6, transcription factor |
| UniProt Gene Name: | POU6F1 |
| UniProt Entry Name: | PO6F1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using POU6F1 antibody (CAB7299) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunofluorescence analysis of A-549 cells using POU6F1 antibody (CAB7299). |
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