Anti-POP7 Antibody (CAB8183)
- SKU:
- CAB8183
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | POP7 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8183 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human POP7 (NP_005828.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | MCF7, SW480 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human POP7 (NP_005828.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAEN REPR GAVE AELD PVEY TLRK RLPS RLPR RPND IYVN MKTD FKAQ LARC QKLL DGGA RGQN ACSE IYIH GLGL AINR AINI ALQL QAGS FGSL QVAA NTST VELV DELE PETD TREP LTRI RNNS AIHI RVFR VTPK |
| Gene ID: | 10248 |
| Uniprot: | O75817 |
| Cellular Location: | Cytoplasm, Cytoplasmic granule, Nucleus, nucleolus |
| Calculated MW: | 15kDa |
| Observed MW: | 14kDa |
| Synonyms: | POP7, 0610037N12Rik, RPP2, RPP20 |
| Background: |
| UniProt Protein Function: | POP7: Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Belongs to the histone-like Alba family. |
| UniProt Protein Details: | Protein type:Nucleolus; Ribonuclease; EC 3.1.26.5 Chromosomal Location of Human Ortholog: 7q22 Cellular Component: nucleolar ribonuclease P complex; nucleus Molecular Function:protein binding; ribonuclease P activity Biological Process: tRNA processing |
| UniProt Code: | O75817 |
| NCBI GenInfo Identifier: | 153791431 |
| NCBI Gene ID: | 10248 |
| NCBI Accession: | NP_005828.2 |
| UniProt Secondary Accession: | O75817,Q9BV74, A4D2E0, |
| UniProt Related Accession: | O75817 |
| Molecular Weight: | 42.7 kDa |
| NCBI Full Name: | ribonuclease P protein subunit p20 |
| NCBI Synonym Full Names: | processing of precursor 7, ribonuclease P/MRP subunit (S. cerevisiae) |
| NCBI Official Symbol: | POP7 |
| NCBI Official Synonym Symbols: | RPP2; RPP20; 0610037N12Rik |
| NCBI Protein Information: | ribonuclease P protein subunit p20; hPOP7; RNaseP protein p20; ribonucleases P/MRP protein subunit POP7 homolog; processing of precursor 7, ribonuclease P subunit; POP7 (processing of precursor, S. cerevisiae) homolog |
| UniProt Protein Name: | Ribonuclease P protein subunit p20 |
| UniProt Synonym Protein Names: | Ribonucleases P/MRP protein subunit POP7 homolog; hPOP7 |
| Protein Family: | Ribonuclease P protein |
| UniProt Gene Name: | POP7 |
| UniProt Entry Name: | POP7_HUMAN |
 | Western blot analysis of extracts of various cell lines, using POP7 antibody (CAB8183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using POP7 antibody (CAB8183) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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