Anti-POLRMT Antibody (CAB15605)
- SKU:
- CAB15605
- Product type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-POLRMT Antibody |
| Antibody SKU: | CAB15605 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100 |
| Reactivity: | Human, Rat |
| Positive Samples: | HeLa, HepG2, 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SSAS PQEQ DQDR RKDW GHVE LLEV LQAR VRQL QAES VSEV VVNR VDVA RLPE CGSG DGSL QPPR KVQM GAKD ATPV PCGR WAKI LEKD KRTQ QMRM QRLK AKLQ MPFQ SGEF KALT RRLQ VEPR LLSK QMAG CLED CTRQ APES PWEE QLAR LLQE APGK LSLD VEQA PSGQ HSQA QLSG QQQR LLAF F |
| Gene ID: | 5442 |
| Uniprot: | O00411 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 138kDa |
| Observed MW: | 139kDa |
| Synonyms: | POLRMT, APOLMT, MTRNAP, MTRPOL, h-mtRPOL |
| Background: | This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. |
| UniProt Protein Function: | DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. |
| NCBI Summary: | This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00411 |
| NCBI GenInfo Identifier: | 110618253 |
| NCBI Gene ID: | 5442 |
| NCBI Accession: | NP_005026.3 |
| UniProt Secondary Accession: | O00411,O60370, |
| UniProt Related Accession: | O00411 |
| Molecular Weight: | 135 |
| NCBI Full Name: | DNA-directed RNA polymerase, mitochondrial |
| NCBI Synonym Full Names: | RNA polymerase mitochondrial |
| NCBI Official Symbol: | POLRMT |
| NCBI Official Synonym Symbols: | APOLMT; MTRNAP; MTRPOL; h-mtRPOL |
| NCBI Protein Information: | DNA-directed RNA polymerase, mitochondrial |
| UniProt Protein Name: | DNA-directed RNA polymerase, mitochondrial |
| UniProt Gene Name: | POLRMT |
 | Western blot analysis of extracts of various cell lines, using POLRMT antibody (CAB15605) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of C6 cells using POLRMT antibody (CAB15605) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using POLRMT antibody (CAB15605) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug POLRMT antibody (CAB15605). Western blot was performed from the immunoprecipitate using POLRMT antibody (CAB15605) at a dilition of 1:1000. |
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