Anti-POLR1B Antibody (CAB12034)
- SKU:
- CAB12034
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-POLR1B Antibody |
| Antibody SKU: | CAB12034 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 670-900 of human POLR1B (NP_061887.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, HT-29, 293T, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 670-900 of human POLR1B (NP_061887.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IANF IPFS DHNQ SPRN MYQC QMGK QTMG FPLL TYQD RSDN KLYR LQTP QSPL VRPS MYDY YDMD NYPI GTNA IVAV ISYT GYDM EDAM IVNK ASWE RGFA HGSV YKSE FIDL SEKI KQGD SSLV FGIK PGDP RVLQ KLDD DGLP FIGA KLQY GDPY YSYL NLNT GESF VMYY KSKE NCVV DNIK VCSN DTGS GKFK CVCI TMRV PRNP TIGD KFAS RHGQ KGIL SRLW PAE |
| Gene ID: | 84172 |
| Uniprot: | Q9H9Y6 |
| Cellular Location: | Nucleus, nucleolus |
| Calculated MW: | 104kDa/107kDa/121kDa/128kDa/132kDa |
| Observed MW: | 128kDa |
| Synonyms: | POLR1B, RPA135, RPA2, Rpo1-2 |
| Background: |
| UniProt Protein Function: | POLR1B: a subunit of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase that synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the clef. Two alternatively spliced human isoforms have been described. |
| UniProt Protein Details: | Protein type:Transferase; Nucleotide Metabolism - purine; EC 2.7.7.6; Transcription initiation complex; Nucleolus; Nucleotide Metabolism - pyrimidine Chromosomal Location of Human Ortholog: 2q13 Cellular Component: cytoplasm; DNA-directed RNA polymerase I complex; nucleolus; nucleoplasm Molecular Function:protein binding Biological Process: positive regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter |
| NCBI Summary: | Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9H9Y6 |
| NCBI GenInfo Identifier: | 92090637 |
| NCBI Gene ID: | 84172 |
| NCBI Accession: | Q9H9Y6.2 |
| UniProt Secondary Accession: | Q9H9Y6,Q2TAM4, Q585T5, Q6ZRR2, Q9H9D3, B7Z6Y7, B7Z823 F5GZX4, F8W898, |
| UniProt Related Accession: | Q9H9Y6 |
| Molecular Weight: | 107,858 Da |
| NCBI Full Name: | DNA-directed RNA polymerase I subunit RPA2 |
| NCBI Synonym Full Names: | RNA polymerase I subunit B |
| NCBI Official Symbol: | POLR1B |
| NCBI Official Synonym Symbols: | RPA2; RPA135; Rpo1-2 |
| NCBI Protein Information: | DNA-directed RNA polymerase I subunit RPA2 |
| UniProt Protein Name: | DNA-directed RNA polymerase I subunit RPA2 |
| UniProt Synonym Protein Names: | DNA-directed RNA polymerase I 135 kDa polypeptide; RPA135 |
| UniProt Gene Name: | POLR1B |
| UniProt Entry Name: | RPA2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using POLR1B antibody (CAB12034) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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