Anti-POLE3 Antibody (CAB6469)[KO Validated]

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SKU:
CAB6469
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-POLE3 Antibody [KO Validated]
Antibody SKU:CAB6469
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human POLE3 (NP_059139.3).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:HepG2, Mouse testis, Mouse bone marrow, Mouse spleen, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human POLE3 (NP_059139.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAER PEDL NLPN AVIT RIIK EALP DGVN ISKE ARSA ISRA ASVF VLYA TSCA NNFA MKGK RKTL NASD VLSA MEEM EFQR FVTP LKEA LEAY RREQ KGKK EASE QKKK DKDK KTDS EEQD KSRD EDND EDEE RLEE EEQN EEEE VDN
Gene ID:54107
Uniprot:Q9NRF9
Cellular Location:Nucleus
Calculated MW:16kDa
Observed MW:20-25kDa
Synonyms:POLE3, CHARAC17, CHRAC17, YBL1, p17
Background:POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]
UniProt Protein Function:POLE3: Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
UniProt Protein Details:

Protein type:Nucleotide Metabolism - pyrimidine; EC 2.7.7.7; DNA replication; Nucleotide Metabolism - purine; Transferase

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: epsilon DNA polymerase complex; nucleus

Molecular Function:protein binding

Biological Process: DNA replication

NCBI Summary:POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]
UniProt Code:Q9NRF9
NCBI GenInfo Identifier:22653710
NCBI Gene ID:54107
NCBI Accession:Q9NRF9.1
UniProt Secondary Accession:Q9NRF9,Q5W0U1, Q8N758, Q8NCE5, Q9NR32,
UniProt Related Accession:Q9NRF9
Molecular Weight:16,860 Da
NCBI Full Name:DNA polymerase epsilon subunit 3
NCBI Synonym Full Names:DNA polymerase epsilon 3, accessory subunit
NCBI Official Symbol:POLE3  
NCBI Official Synonym Symbols:p17; YBL1; CHRAC17; CHARAC17  
NCBI Protein Information:DNA polymerase epsilon subunit 3
UniProt Protein Name:DNA polymerase epsilon subunit 3
UniProt Synonym Protein Names:Arsenic-transactivated protein; AsTP; Chromatin accessibility complex 17 kDa protein; CHRAC-17; HuCHRAC17; DNA polymerase II subunit 3; DNA polymerase epsilon subunit p17
Protein Family:DNA polymerase
UniProt Gene Name:POLE3  
UniProt Entry Name:DPOE3_HUMAN
Anti-POLE3 Antibody [KO Validated] (CAB6469)Western blot analysis of extracts of various cell lines, using POLE3 antibody (CAB6469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-POLE3 Antibody [KO Validated] (CAB6469)Western blot analysis of extracts from normal (control) and [KO Validated] POLE3 Rabbit pAb knockout (KO) 293T cells, using [KO Validated] POLE3 Rabbit pAb antibody (CAB6469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-POLE3 Antibody [KO Validated] (CAB6469)Immunofluorescence analysis of U2OS cells using POLE3 antibody (CAB6469).
Anti-POLE3 Antibody [KO Validated] (CAB6469)Immunofluorescence analysis of U2OS cells using POLE3 antibody (CAB6469). (Green)gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.

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