Anti-POFUT2 Antibody (CAB12223)
- SKU:
- CAB12223
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-POFUT2 Antibody |
| Antibody SKU: | CAB12223 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-370 of human POFUT2 (NP_056042.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, HepG2, Mouse brain, Mouse heart, Rat brain, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-370 of human POFUT2 (NP_056042.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVFA RHLR EVGD EFRS RHLN STDD ADRI PFQE DWMK MKVK LGSA LGGP YLGV HLRR KDFI WGHR QDVP SLEG AVRK IRSL MKTH RLDK VFVA TDAV RKEY EELK KLLP EMVR FEPT WEEL ELYK DGGV AII |
| Gene ID: | 23275 |
| Uniprot: | Q9Y2G5 |
| Cellular Location: | Endoplasmic reticulum, Golgi apparatus |
| Calculated MW: | 44kDa/48kDa/49kDa |
| Observed MW: | 50kDa |
| Synonyms: | POFUT2, C21orf80, FUT13 |
| Background: |
| UniProt Protein Function: | POFUT2: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in thrombospondin type 1 repeats. Belongs to the glycosyltransferase 68 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.4.1.221; Transferase Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: endoplasmic reticulum membrane; Golgi apparatus Molecular Function:peptide-O-fucosyltransferase activity Biological Process: regulation of secretion |
| NCBI Summary: | Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9Y2G5 |
| NCBI GenInfo Identifier: | 59803123 |
| NCBI Gene ID: | 23275 |
| NCBI Accession: | Q9Y2G5.3 |
| UniProt Secondary Accession: | Q9Y2G5,Q6PJV1, Q7Z4N0, Q8WWU6, Q9BQS4, Q9BQS5, Q9UFY3 |
| UniProt Related Accession: | Q9Y2G5 |
| Molecular Weight: | 50kDa |
| NCBI Full Name: | GDP-fucose protein O-fucosyltransferase 2 |
| NCBI Synonym Full Names: | protein O-fucosyltransferase 2 |
| NCBI Official Symbol: | POFUT2 |
| NCBI Official Synonym Symbols: | FUT13; C21orf80 |
| NCBI Protein Information: | GDP-fucose protein O-fucosyltransferase 2 |
| UniProt Protein Name: | GDP-fucose protein O-fucosyltransferase 2 |
| UniProt Synonym Protein Names: | Peptide-O-fucosyltransferase 2; O-FucT-2 |
| UniProt Gene Name: | POFUT2 |
 | Western blot analysis of extracts of various cell lines, using POFUT2 antibody (CAB12223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of H9C2 cells using POFUT2 Rabbit pAb (CAB12223) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using POFUT2 Rabbit pAb (CAB12223) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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