Anti-PLZF Antibody (CAB5863)
- SKU:
- CAB5863
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-PLZF Antibody |
| Antibody SKU: | CAB5863 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PLZF (NP_001018011.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Mouse lung, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PLZF (NP_001018011.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDLT KMGM IQLQ NPSH PTGL LCKA NQMR LAGT LCDV VIMV DSQE FHAH RTVL ACTS KMFE ILFH RNSQ HYTL DFLS PKTF QQIL EYAY TATL QAKA EDLD DLLY AAEI LEIE YLEE QCLK MLET IQAS DDND TEAT MADG GAEE EEDR KARY LKNI FISK HSSE ESGY ASVA GQSL PGPM VDQS PSVS TSFG LSAM SPTK AAVD SLMT IGQS LLQG TLQP |
| Gene ID: | 7704 |
| Uniprot: | Q05516 |
| Cellular Location: | Nucleus |
| Calculated MW: | 61kDa/74kDa |
| Observed MW: | 80kDa |
| Synonyms: | ZBTB16, PLZF, ZNF145 |
| Background: | This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized. |
| UniProt Protein Function: | PLZF: Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Defects in ZBTB16 are the cause of skeletal defects genital hypoplasia and mental retardation (SGYMR). A disorder characterized by mental retardation, craniofacial dysmorphism, microcephaly and short stature. Additional features include absence of the thumbs, hypoplasia of the radii and ulnae, additional vertebrae and ribs, retarded bone age and genital hypoplasia. A chromosomal aberration involving ZBTB16 may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with RARA. Belongs to the krueppel C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; DNA-binding; Oncoprotein; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 11q23.1 Cellular Component: nuclear body; PML body; transcriptional repressor complex; nucleolus; plasma membrane; nuclear speck; nucleus; cytosol Molecular Function:protein C-terminus binding; identical protein binding; protein domain specific binding; protein binding; protein homodimerization activity; DNA binding; metal ion binding; double-stranded DNA binding Biological Process: central nervous system development; negative regulation of myeloid cell differentiation; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; positive regulation of transcription, DNA-dependent; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; embryonic pattern specification; embryonic hindlimb morphogenesis; positive regulation of ossification; anterior/posterior pattern formation; forelimb morphogenesis; negative regulation of cell proliferation; positive regulation of chondrocyte differentiation; cartilage development; positive regulation of fat cell differentiation; mesonephros development; myeloid cell differentiation; positive regulation of NK T cell differentiation; hemopoiesis; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; male germ-line stem cell division Disease: Skeletal Defects, Genital Hypoplasia, And Mental Retardation |
| NCBI Summary: | This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q05516 |
| NCBI GenInfo Identifier: | 90109930 |
| NCBI Gene ID: | 7704 |
| NCBI Accession: | Q05516.2 |
| UniProt Secondary Accession: | Q05516,Q8TAL4, |
| UniProt Related Accession: | Q05516 |
| Molecular Weight: | |
| NCBI Full Name: | Zinc finger and BTB domain-containing protein 16 |
| NCBI Synonym Full Names: | zinc finger and BTB domain containing 16 |
| NCBI Official Symbol: | ZBTB16 |
| NCBI Official Synonym Symbols: | PLZF; ZNF145 |
| NCBI Protein Information: | zinc finger and BTB domain-containing protein 16; zinc finger protein PLZF; promyelocytic leukaemia zinc finger; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia) |
| UniProt Protein Name: | Zinc finger and BTB domain-containing protein 16 |
| UniProt Synonym Protein Names: | Promyelocytic leukemia zinc finger protein; Zinc finger protein 145; Zinc finger protein PLZF |
| Protein Family: | Zinc finger and BTB domain-containing protein |
| UniProt Gene Name: | ZBTB16 |
| UniProt Entry Name: | ZBT16_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PLZF Antibody (CAB5863) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded Mouse lung using PLZF antibody (CAB5863) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human colon using PLZF antibody (CAB5863) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat brain using PLZF antibody (CAB5863) at dilution of 1:200 (40x lens). |
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