Anti-PLS3 Antibody (CAB3627)
- SKU:
- CAB3627
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PLS3 Antibody |
| Antibody SKU: | CAB3627 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PLS3 (NP_005023.2). |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, MCF-7, BT-474, A-431, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PLS3 (NP_005023.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDEM ATTQ ISKD ELDE LKEA FAKV DLNS NGFI CDYE LHEL FKEA NMPL PGYK VREI IQKL MLDG DRNK DGKI SFDE FVYI FQEV KSSD IAKT FRKA INRK EGIC ALGG TSEL SSEG TQHS YSEE EKYA FVNW INKA LEND PDCR HVIP MNPN TDDL FKAV GDGI VLCK MINL SVPD TIDE RAIN KKKL TPFI IQEN LNLA LNSA SAIG CHVV NIGA EDLR AGKP HLVL GLLW QIIK IGLF |
| Gene ID: | 5358 |
| Uniprot: | P13797 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 65kDa/69kDa/70kDa |
| Observed MW: | 71kDa |
| Synonyms: | PLS3, BMND18, T-plastin, plastin-3 |
| Background: | Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | PLS3: Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. |
| UniProt Protein Details: | Protein type:Actin-binding; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: Xq23 Cellular Component: cytoplasm Molecular Function:calcium ion binding; actin binding Disease: Bone Mineral Density Quantitative Trait Locus 18 |
| UniProt Code: | P13797 |
| NCBI GenInfo Identifier: | 226694201 |
| NCBI Gene ID: | |
| NCBI Accession: | P13797.4 |
| Molecular Weight: | ~ 71kDa |
| NCBI Full Name: | Plastin-3 |
| UniProt Protein Name: | Plastin-3 |
| UniProt Synonym Protein Names: | T-plastin |
| Protein Family: | Plastin |
| UniProt Gene Name: | PLS3 |
| UniProt Entry Name: | PLST_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PLS3 antibody (CAB3627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human stomach using PLS3 antibody (CAB3627) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug PLS3 antibody (CAB3627). Western blot was performed from the immunoprecipitate using PLS3 antibody (CAB3627) at a dilition of 1:1000. |
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