Anti-PLEKHG4 Antibody (CAB16112)
- SKU:
- CAB16112
- Product type:
- Antibody
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PLEKHG4 Antibody |
| Antibody SKU: | CAB16112 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 710-1010 of human PLEKHG4 (NP_001123199.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Rat |
| Positive Samples: | Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 710-1010 of human PLEKHG4 (NP_001123199.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GALP QASP TVPP PGSS DPRS LNRL QLVL AEMV ATER EYVR ALEY TMEN YFPE LDRP DVPQ GLRG QRAH LFGN LEKL RDFH CHFF LREL EACT RHPP RVAY AFLR HRVQ FGMY ALYS KNKP RSDA LMSS YGHT FFKD KQQA LGDH LDLA SYLL KPIQ RMGK YALL LQEL ARAC GGPT QELS ALRE AQSL VHFQ LRHG NDLL AMDA IQGC DVNL KEQG QLVR QDEF VVRT GRHK SVRR IFLF EELL LFSK PRHG PTGV DTFA YKRS FKMA DLGL TECC GNSN LRFE IWFR RRKA RDTF VLQA S |
| Gene ID: | 25894 |
| Uniprot: | Q58EX7 |
| Cellular Location: | |
| Calculated MW: | 20kDa/122kDa/130kDa |
| Observed MW: | 131kDa |
| Synonyms: | PLEKHG4, ARHGEF44, PRTPHN1, SCA4 |
| Background: | The protein encoded by this gene can function as a guanine nucleotide exchange factor (GEF) and may play a role in intracellular signaling and cytoskeleton dynamics at the Golgi apparatus. Polymorphisms in the region of this gene have been found to be associated with spinocerebellar ataxia in some study populations. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | PLEKHG4: a guanine nucleotide exchange factor (GEF) that facilitates activation of the small GTPases Rac1, Cdc42, and RhoA. A member of the Dbl family GEFs that regulates GTPase signaling critical for proper cerebellar function. Its expression is developmentally regulated in the murine brain, with pronounced expression in the newborn midbrain and brainstem that wanes with age and maximal expression in adult cerebellar Purkinje neurons. Widely expressed at low levels. More strongly expressed in testis and pancreas. Expressed in kidney, Leydig cells in the testis, epithelial cells in the prostate gland and Langerhans islet in the pancreas. May play a role in cytoskeleton dynamics at the Golgi. Overexpression in NIH3T3 cells induces rearrangements of the actin cytoskeleton including enhanced formation of lamellopodia and fillopodia. Three isoforms of the human protein are produced by alternative splicing. Isoform 1 and isoform 3 are strongly expressed in Purkinje cells and to a lower extent in other neurons. |
| UniProt Protein Details: | Protein type:GEFs; GEFs, Rac/Rho Chromosomal Location of Human Ortholog: 16q22.1 Molecular Function:protein binding; Rho guanyl-nucleotide exchange factor activity Biological Process: regulation of Rho protein signal transduction Disease: Spinocerebellar Ataxia 31; Spinocerebellar Ataxia 4 |
| NCBI Summary: | The protein encoded by this gene can function as a guanine nucleotide exchange factor (GEF) and may play a role in intracellular signaling and cytoskeleton dynamics at the Golgi apparatus. Polymorphisms in the region of this gene have been found to be associated with spinocerebellar ataxia in some study populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
| UniProt Code: | Q58EX7 |
| NCBI GenInfo Identifier: | 74755121 |
| NCBI Gene ID: | 25894 |
| NCBI Accession: | Q58EX7.1 |
| UniProt Secondary Accession: | Q58EX7,Q4G0J8, Q4H485, Q56A69, Q9H7K4, Q9UFW0, |
| UniProt Related Accession: | Q58EX7 |
| Molecular Weight: | 20,247 Da |
| NCBI Full Name: | Puratrophin-1 |
| NCBI Synonym Full Names: | pleckstrin homology and RhoGEF domain containing G4 |
| NCBI Official Symbol: | PLEKHG4 |
| NCBI Official Synonym Symbols: | SCA4; PRTPHN1; ARHGEF44 |
| NCBI Protein Information: | puratrophin-1 |
| UniProt Protein Name: | Puratrophin-1 |
| UniProt Synonym Protein Names: | Pleckstrin homology domain-containing family G member 4; PH domain-containing family G member 4; Purkinje cell atrophy-associated protein 1 |
| Protein Family: | Puratrophin |
| UniProt Gene Name: | PLEKHG4 |
| UniProt Entry Name: | PKHG4_HUMAN |
 | Western blot analysis of extracts of rat testis, using PLEKHG4 antibody (CAB16112) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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