Anti-PLCD4 Antibody (CAB7841)
- SKU:
- CAB7841
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-PLCD4 Antibody |
| Antibody SKU: | CAB7841 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PLCD4 (NP_116115.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BT-474, THP-1, Mouse brain, Mouse testis, Mouse spinal cord, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PLCD4 (NP_116115.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASL LQDQ LTTD QDLL LMQE GMPM RKVR SKSW KKLR YFRL QNDG MTVW HARQ ARGS AKPS FSIS DVET IRNG HDSE LLRS LAEE LPLE QGFT IVFH GRRS NLDL MANS VEEA QIWM RGLQ LLVD LVTS MDHQ ERLD QWLS DWFQ RGDK NQDG KMSF QEVQ RLLH LMNV EMDQ EYAF SLFQ AADT SQSG TLEG EEFV QFYK ALTK RAEV QELF ESFS ADGQ KLTL LEFL DFLQ EEQK ERDC TSEL ALEL IDRY EPSD SGKL RHVL SMDG FLSY LCSK DGDI FNPA CLPI YQ |
| Gene ID: | 84812 |
| Uniprot: | Q9BRC7 |
| Cellular Location: | Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus, Peripheral membrane protein |
| Calculated MW: | 31kDa/87kDa |
| Observed MW: | 110kDa |
| Synonyms: | PLCD4 |
| Background: | This gene encodes a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4, 5-bisphosphate into two intracellular second messengers, inositol 1, 4, 5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer. |
| UniProt Protein Function: | PLCD4: Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida- induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Phospholipase; EC 3.1.4.11; Carbohydrate Metabolism - inositol phosphate Chromosomal Location of Human Ortholog: 2q35 Cellular Component: nuclear membrane; endoplasmic reticulum; cytoplasm; plasma membrane Molecular Function:signal transducer activity; calcium ion binding; phosphoinositide phospholipase C activity Biological Process: acrosome reaction; phosphatidylinositol metabolic process; lipid catabolic process |
| NCBI Summary: | This gene encodes a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer. [provided by RefSeq, Jan 2011] |
| UniProt Code: | Q9BRC7 |
| NCBI GenInfo Identifier: | 74732863 |
| NCBI Gene ID: | 84812 |
| NCBI Accession: | Q9BRC7.1 |
| UniProt Secondary Accession: | Q9BRC7,Q53FS8, |
| UniProt Related Accession: | Q9BRC7 |
| Molecular Weight: | 31,448 Da |
| NCBI Full Name: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 |
| NCBI Synonym Full Names: | phospholipase C, delta 4 |
| NCBI Official Symbol: | PLCD4 |
| NCBI Protein Information: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4; PLC delta4; phosphoinositide phospholipase C-delta-4; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-4 |
| UniProt Protein Name: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 |
| UniProt Synonym Protein Names: | Phosphoinositide phospholipase C-delta-4; Phospholipase C-delta-4; PLC-delta-4 |
| Protein Family: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase |
| UniProt Gene Name: | PLCD4 |
| UniProt Entry Name: | PLCD4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PLCD4 antibody (CAB7841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of U2OS cells using PLCD4 antibody (CAB7841) at dilution of 1:100. Blue: DAPI for nuclear staining. |