Anti-PLB1 Antibody (CAB10429)
- SKU:
- CAB10429
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-PLB1 Antibody |
| Antibody SKU: | CAB10429 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1110-1415 of human PLB1 (NP_694566.4). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-431, U-87MG, HL-60, HT-29, Mouse testis, Mouse lung, Mouse skeletal muscle, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1110-1415 of human PLB1 (NP_694566.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ARPN NSSD LPTS WRGL SWSI GGDG NLET HTTL PNIL KKFN PYLL GFST STWE GTAG LNVA AEGA RARD MPAQ AWDL VERM KNSP DINL EKDW KLVT LFIG VNDL CHYC ENPE AHLA TEYV QHIQ QALD ILSE ELPR AFVN VVEV MELA SLYQ GQGG KCAM LAAQ NNCT CLRH SQSS LEKQ ELKK VNWN LQHG ISSF SYWH QYTQ REDF AVVV QPFF QNTL TPLN ERGD TDLT FFSE DCFH FSDR GHAE MAIA LWNN MLEP VGRK TTSN NFTH SRAK LKCP SPES PYLY TLRN SRLL PDQA EEAP EV |
| Gene ID: | 151056 |
| Uniprot: | Q6P1J6 |
| Cellular Location: | Apical cell membrane, Single-pass type I membrane protein |
| Calculated MW: | 47kDa/54kDa/85kDa/161kDa/163kDa |
| Observed MW: | 170kDa |
| Synonyms: | PLB1, PLB, PLB/LIP, hPLB |
| Background: | This gene encodes a membrane-associated phospholipase that displays lysophospholipase and phospholipase A2 activities through removal of sn-1 and sn-2 fatty acids of glycerophospholipids. In addition, it displays lipase and retinyl ester hydrolase activities. The encoded protein is highly conserved and is composed of a large, glycosylated extracellular domain composed of four tandem homologous domains, followed by a hydrophobic segment that anchors the enzyme to the membrane and a short C-terminal cytoplasmic tail. This gene has been identified as a candidate rheumatoid arthritis risk gene. |
| UniProt Protein Function: | PLB1: Membrane-associated phospholipase. Exhibits a calcium- independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids. Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.1.1.4; EC 3.1.1.5; Membrane protein, integral; Phospholipase Chromosomal Location of Human Ortholog: 2p23.2 Cellular Component: plasma membrane Molecular Function:phospholipase A2 activity; retinyl-palmitate esterase activity Biological Process: retinoid metabolic process |
| NCBI Summary: | This gene encodes a membrane-associated phospholipase that displays lysophospholipase and phospholipase A2 activities through removal of sn-1 and sn-2 fatty acids of glycerophospholipids. In addition, it displays lipase and retinyl ester hydrolase activities. The encoded protein is highly conserved and is composed of a large, glycosylated extracellular domain composed of four tandem homologous domains, followed by a hydrophobic segment that anchors the enzyme to the membrane and a short C-terminal cytoplasmic tail. This gene has been identified as a candidate rheumatoid arthritis risk gene. [provided by RefSeq, Jul 2016] |
| UniProt Code: | Q6P1J6 |
| NCBI GenInfo Identifier: | 296452994 |
| NCBI Gene ID: | 151056 |
| NCBI Accession: | Q6P1J6.3 |
| UniProt Secondary Accession: | Q6P1J6,Q53S03, Q8IUP7, Q96DP9, A8KAX2, |
| UniProt Related Accession: | Q6P1J6 |
| Molecular Weight: | 163kDa |
| NCBI Full Name: | Phospholipase B1, membrane-associated |
| NCBI Synonym Full Names: | phospholipase B1 |
| NCBI Official Symbol: | PLB1 |
| NCBI Official Synonym Symbols: | PLB; PLB/LIP |
| NCBI Protein Information: | phospholipase B1, membrane-associated |
| UniProt Protein Name: | Phospholipase B1, membrane-associated |
| UniProt Synonym Protein Names: | Phospholipase B/lipase; PLB/LIP |
| Protein Family: | Phospholipase |
| UniProt Gene Name: | PLB1 |
 | Western blot analysis of extracts of various cell lines, using PLB1 antibody (CAB10429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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