Anti-PIK3CG 항체 (CAB6688)

Antibody Name:	Anti-PIK3CG Antibody
Antibody SKU:	CAB6688
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PIK3CG (NP_002640.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, K-562, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PIK3CG (NP_002640.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MELE NYKQ PVVL REDN CRRR RRMK PRSA AASL SSME LIPI EFVL PTSQ RKCK SPET ALLH VAGH GNVE QMKA QVWL RALE TSVA ADFY HRLG PHHF LLLY QKKG QWYE IYDK YQVV QTLD CLRY WKAT HRSP GQIH LVQR HPPS EESQ AFQR QLTA LIGY DVTD VSNV HDDE LEFT RRGL VTPR MAEV ASRD PKLY AMHP
Gene ID:	5294
Uniprot:	P48736
Cellular Location:	Cell membrane, Cytoplasm
Calculated MW:	126kDa
Observed MW:	110KDa

Synonyms:

PIK3CG, PI3CG, PI3K, PI3Kgamma, PIK3, p110gamma, p120-PI3K

Background:

Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	PIK3CG: catalytic subunit of the PI3/PI4-kinase family of phospholipid-kinases. Phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. Phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 recruits proteins with PH domains to the membrane, including AKT1 and PDPK1, and plays a critical pleiotropic role in regulating membrane signaling. Activated by both the alpha and the beta:gamma G proteins following stimulation of G protein-coupled receptors (GPCRs), linking GPCR activation to PIP3 production. Activation by GPCRs is assisted by the regulatory subunits (PIK3R5 or PIK3R6), leading to the translocation from the cytosol to the plasma membrane. Inhibited by AS-604850 and AS-605240. Activates signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in immune, inflammatory and allergic responses. Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge. Controls motility of dendritic cells. Together with PIK3CD, it regulates many cellular functions: it is involved in natural killer (NK) cell development and migration towards the sites of inflammation; participates in T-lymphocyte development and migration; required for B-lymphocyte development and signaling; regulates lymphocyte cytokine production; participates in neutrophil respiratory burst, chemotaxis and extravasation; promotes platelet aggregation and thrombosis; regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism; involved in endothelial progenitor cell migration; modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels; regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to ADRBK1 and by non-muscle tropomyosin phosphorylation; and contributes to cardiac hypertrophy under pathological stress. Possesses serine/threonine protein kinase activity: both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis. Holoenzyme is a trimer composed of a heterodimer of a catalytic subunit PIK3CG and a PIK3R5 or PIK3R6 regulatory subunit. Interacts with HRAS1; the interaction is required for membrane recruitment and beta-gamma G protein dimer-dependent activation of the PI3K gamma complex PIK3CG:PIK3R6.
UniProt Protein Details:	Protein type:EC 2.7.11.1; Motility/polarity/chemotaxis; Autophagy; EC 2.7.1.153; Carbohydrate Metabolism - inositol phosphate; Kinase, lipid Chromosomal Location of Human Ortholog: 7q22.3 Cellular Component: cytoplasm; cytosol; mast cell granule; membrane; plasma membrane Molecular Function:1-phosphatidylinositol-3-kinase activity; ATP binding; ephrin receptor binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity Biological Process: adaptive immune response; angiogenesis; blood coagulation; cytokine production; dendritic cell chemotaxis; elevation of cytosolic calcium ion concentration; endocytosis; G-protein coupled receptor protein signaling pathway; inflammatory response; innate immune response; mast cell degranulation; neutrophil chemotaxis; phosphatidylinositol biosynthetic process; phosphoinositide 3-kinase cascade; phosphoinositide phosphorylation; phospholipid metabolic process; platelet activation; positive regulation of acute inflammatory response; positive regulation of MAP kinase activity; positive regulation of protein kinase B signaling cascade; protein amino acid phosphorylation; regulation of cell adhesion mediated by integrin; respiratory burst during defense response; secretory granule localization; T cell activation; T cell proliferation
NCBI Summary:	Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2015]
UniProt Code:	P48736
NCBI GenInfo Identifier:	92090623
NCBI Gene ID:	5294
NCBI Accession:	P48736.3
UniProt Secondary Accession:	P48736,Q8IV23, Q9BZC8, A4D0Q6,
UniProt Related Accession:	P48736
Molecular Weight:	126,454 Da
NCBI Full Name:	Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
NCBI Synonym Full Names:	phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma
NCBI Official Symbol:	PIK3CG
NCBI Official Synonym Symbols:	PI3K; PIK3; PI3CG; PI3Kgamma; p110gamma; p120-PI3K
NCBI Protein Information:	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
UniProt Protein Name:	Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
UniProt Synonym Protein Names:	Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma; PtdIns-3-kinase subunit p110-gamma; p110gamma; Phosphoinositide-3-kinase catalytic gamma polypeptide; Serine/threonine protein kinase PIK3CG (EC:2.7.11.1); p120-PI3K
Protein Family:	Phosphatidylinositol 4,5-bisphosphate 3-kinase
UniProt Gene Name:	PIK3CG
UniProt Entry Name:	PK3CG_HUMAN

	Western blot analysis of extracts of various cell lines, using PIK3CG antibody (CAB6688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded rat spleen using PIK3CG Rabbit pAb (CAB6688) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human thyroid cancer using PIK3CG Rabbit pAb (CAB6688) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using PIK3CG Rabbit pAb (CAB6688) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using PIK3CG Rabbit pAb (CAB6688) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.