Anti-PIK3CA Antibody (CAB0265)

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SKU:
CAB0265
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular
Frequently bought together:

Description

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Antibody Name:Anti-PIK3CA Antibody
Antibody SKU:CAB0265
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 401-600 of human PIK3CA (NP_006209.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 401-600 of human PIK3CA (NP_006209.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RLCL SICS VKGR KGAK EEHC PLAW GNIN LFDY TDTL VSGK MALN LWPV PHGL EDLL NPIG VTGS NPNK ETPC LELE FDWF SSVV KFPD MSVI EEHA NWSV SREA GFSY SHAG LSNR LARD NELR ENDK EQLK AIST RDPL SEIT EQEK DFLW SHRH YCVT IPEI LPKL LLSV KWNS RDEV AQMY CLVK DWPP IKPE QAME
Gene ID:5290
Uniprot:P42336
Cellular Location:
Calculated MW:124kDa
Observed MW:115kDa
Synonyms:PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha
Background:Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4, 5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.
UniProt Protein Function:PIK3CA: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1- AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with IRS1 in nuclear extracts. Interacts with RUFY3. Interacts with RASD2. Interacts with APPL1. Interacts with HRAS1 and KRAS. Interaction with HRAS1/KRAS is required for PI3K pathway signaling and cell proliferation stimulated by EGF and FGF2. Belongs to the PI3/PI4-kinase family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Oncoprotein; EC 2.7.11.1; EC 2.7.1.153; Kinase, lipid; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 3q26.3

Cellular Component: lamellipodium; plasma membrane; phosphoinositide 3-kinase complex; cytosol

Molecular Function:protein serine/threonine kinase activity; protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; insulin receptor substrate binding; 1-phosphatidylinositol-3-kinase activity; kinase activity; protein kinase activator activity; ATP binding; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; regulation of multicellular organism growth; glucose metabolic process; positive regulation of peptidyl-serine phosphorylation; T cell receptor signaling pathway; protein amino acid phosphorylation; vasculature development; phosphoinositide phosphorylation; positive regulation of protein kinase activity; phospholipid metabolic process; T cell costimulation; phosphatidylinositol biosynthetic process; protein kinase B signaling cascade; insulin receptor signaling pathway; innate immune response; endothelial cell migration; negative regulation of neuron apoptosis; angiogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration; cardiac muscle contraction

Disease: Cowden Syndrome 5; Gastric Cancer; Keratosis, Seborrheic; Breast Cancer; Lung Cancer; Ovarian Cancer; Congenital Lipomatous Overgrowth, Vascular Malformations, And Epidermal Nevi; Colorectal Cancer; Megalencephaly-capillary Malformation-polymicrogyria Syndrome; Hepatocellular Carcinoma; Nevus, Epidermal

NCBI Summary:Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. [provided by RefSeq, Apr 2016]
UniProt Code:P42336
NCBI GenInfo Identifier:126302584
NCBI Gene ID:5290
NCBI Accession:P42336.2
UniProt Related Accession:P42336
Molecular Weight:~ 110kDa
NCBI Full Name:Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
NCBI Synonym Full Names:phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
NCBI Official Symbol:PIK3CA  
NCBI Official Synonym Symbols:MCM; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-alpha  
NCBI Protein Information:phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
UniProt Protein Name:Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
UniProt Synonym Protein Names:Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA (EC:2.7.11.1)
UniProt Gene Name:PIK3CA  
UniProt Entry Name:PK3CA_HUMAN
Anti-PIK3CA Antibody (CAB0265)Western blot analysis of extracts of HeLa cells, using PIK3CA antibody (CAB0265) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-PIK3CA Antibody (CAB0265)Immunohistochemistry of paraffin-embedded rat kidney using PIK3CA antibody (CAB0265) at dilution of 1:100 (40x lens).
Anti-PIK3CA Antibody (CAB0265)Immunohistochemistry of paraffin-embedded human colon carcinoma using PIK3CA antibody (CAB0265) at dilution of 1:100 (40x lens).
Anti-PIK3CA Antibody (CAB0265)Immunohistochemistry of paraffin-embedded mouse kidney using PIK3CA antibody (CAB0265) at dilution of 1:100 (40x lens).
Anti-PIK3CA Antibody (CAB0265)Immunofluorescence analysis of HeLa cells using PIK3CA antibody (CAB0265) at dilution of 1:100. Blue: DAPI for nuclear staining.

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