Anti-PIK3C3 Antibody (CAB4021)

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SKU:
CAB4021
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Autophagy
Frequently bought together:

Description

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Antibody Name:Anti-PIK3C3 Antibody
Antibody SKU:CAB4021
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide of human PIK3C3
Application:WB IHC IF
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa, PC-3, Mouse brain
Immunogen:A synthetic peptide of human PIK3C3
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5289
Uniprot:Q8NEB9
Cellular Location:Cytoplasmic vesicle, Late endosome, Midbody, autophagosome
Calculated MW:101kDa
Observed MW:100kDa
Synonyms:PIK3C3, VPS34, Vps34, hVps34
Background:
UniProt Protein Function:PIK3C3: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes. Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive. Part of a complex composed of PIK3R4 and PIK3CB. Interacts with RAB7A in the presence of PIK3R4. Ubiquitously expressed, with a highest expression in skeletal muscle. Belongs to the PI3/PI4-kinase family.
UniProt Protein Details:

Protein type:EC 2.7.1.137; Autophagy; Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; Kinase, lipid

Chromosomal Location of Human Ortholog: 18q12.3

Cellular Component: membrane; late endosome; phagocytic vesicle; midbody; axoneme; phosphoinositide 3-kinase complex; cytosol

Molecular Function:protein binding; 1-phosphatidylinositol-3-kinase activity; ATP binding; protein kinase activity

Biological Process: phosphoinositide-mediated signaling; endosome organization and biogenesis; cytokinesis; protein amino acid phosphorylation; regulation of protein secretion; response to leucine; phosphoinositide phosphorylation; early endosome to late endosome transport; phospholipid metabolic process; phosphatidylinositol biosynthetic process; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; protein processing; toll-like receptor 9 signaling pathway; autophagic vacuole formation

UniProt Code:Q8NEB9
NCBI GenInfo Identifier:74730233
NCBI Gene ID:5289
NCBI Accession:Q8NEB9.1
UniProt Secondary Accession:Q8NEB9,Q15134,
UniProt Related Accession:Q8NEB9
Molecular Weight:101,549 Da
NCBI Full Name:Phosphatidylinositol 3-kinase catalytic subunit type 3
NCBI Synonym Full Names:phosphatidylinositol 3-kinase, catalytic subunit type 3
NCBI Official Symbol:PIK3C3  
NCBI Official Synonym Symbols:VPS34; Vps34; hVps34  
NCBI Protein Information:phosphatidylinositol 3-kinase catalytic subunit type 3
UniProt Protein Name:Phosphatidylinositol 3-kinase catalytic subunit type 3
UniProt Synonym Protein Names:Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34
UniProt Gene Name:PIK3C3  
UniProt Entry Name:PK3C3_HUMAN
Anti-PIK3C3 Antibody (CAB4021)Western blot analysis of extracts of various cell lines, using PIK3C3 antibody (CAB4021). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

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