Anti-PIGX Antibody (CAB15460)
- SKU:
- CAB15460
- Product type:
- Antibody
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-PIGX Antibody |
| Antibody SKU: | CAB15460 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-220 of human PIGX (NP_060331.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Rat |
| Positive Samples: | Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-220 of human PIGX (NP_060331.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LIKQ DIPA GLYV DPYE LASL RERN ITEA VMVS ENFD IEAP NYLS KESE VLIY ARRD SQCI DCFQ AFLP VHCR YHRP HSED GEAS IVVN NPDL LMFC DQEF PILK CWAH SEVA APCA LENE DICQ WNKM KYKS VYKN VILQ V |
| Gene ID: | 54965 |
| Uniprot: | Q8TBF5 |
| Cellular Location: | Endoplasmic reticulum membrane, Single-pass type I membrane protein |
| Calculated MW: | 28kDa/30kDa |
| Observed MW: | 29kDa |
| Synonyms: | PIGX, PIG-X |
| Background: | This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | Essential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM. |
| NCBI Summary: | This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q8TBF5 |
| NCBI GenInfo Identifier: | 261490708 |
| NCBI Gene ID: | 54965 |
| NCBI Accession: | NP_001159776.1 |
| UniProt Secondary Accession: | Q8TBF5,Q9NWZ2, |
| UniProt Related Accession: | Q8TBF5 |
| Molecular Weight: | 30,974 Da |
| NCBI Full Name: | phosphatidylinositol-glycan biosynthesis class X protein isoform 1 |
| NCBI Synonym Full Names: | phosphatidylinositol glycan anchor biosynthesis class X |
| NCBI Official Symbol: | PIGX |
| NCBI Official Synonym Symbols: | PIG-X |
| NCBI Protein Information: | phosphatidylinositol-glycan biosynthesis class X protein |
| UniProt Protein Name: | Phosphatidylinositol-glycan biosynthesis class X protein |
| Protein Family: | Phosphatidylinositol-glycan biosynthesis class X protein |
| UniProt Gene Name: | PIGX |
 | Western blot analysis of extracts of rat kidney, using PIGX antibody (CAB15460) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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