Anti-PIGT Antibody (CAB12119)
- SKU:
- CAB12119
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-PIGT Antibody |
| Antibody SKU: | CAB12119 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human PIGT (NP_057021.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, A-431, LO2, B cells, Mouse brain, Mouse testis, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human PIGT (NP_057021.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LFRM FSRT LTEP CPLA SESR VYVD ITTY NQDN ETLE VHPP PTTT YQDV ILGT RKTY AIYD LLDT AMIN NSRN LNIQ LKWK RPPE NEAP PVPF LHAQ RYVS GYGL QKGE LSTL LYNT HPYR AFPV LLLD TVPW YLRL YVHT LTIT SKGK ENKP SYIH YQPA QDRL QPHL LEML IQLP ANSV TKVS IQFE RALL KWTE YTPD P |
| Gene ID: | 51604 |
| Uniprot: | Q969N2 |
| Cellular Location: | Endoplasmic reticulum membrane, Single-pass type I membrane protein |
| Calculated MW: | 41kDa/43kDa/54kDa/57kDa/59kDa/65kDa |
| Observed MW: | 66kDa |
| Synonyms: | PIGT, CGI-06, MCAHS3, NDAP, PNH2 |
| Background: | This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | PIGT: Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates. Belongs to the PIGT family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Membrane protein, integral Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: endoplasmic reticulum membrane; GPI-anchor transamidase complex; membrane Molecular Function:GPI-anchor transamidase activity; protein binding Biological Process: attachment of GPI anchor to protein Disease: Multiple Congenital Anomalies-hypotonia-seizures Syndrome 3; Paroxysmal Nocturnal Hemoglobinuria 2 |
| NCBI Summary: | This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
| UniProt Code: | Q969N2 |
| NCBI GenInfo Identifier: | 44888284 |
| NCBI Gene ID: | 51604 |
| NCBI Accession: | Q969N2.1 |
| UniProt Secondary Accession: | Q969N2,Q2NL69, Q7Z3N7, Q9BQY7, Q9BQY8, Q9UJG6, Q9Y2Z5 B2RND5, B7Z3N1, B7Z7I8, E1P622, G8JLF5, |
| UniProt Related Accession: | Q969N2 |
| Molecular Weight: | 66kDa |
| NCBI Full Name: | GPI transamidase component PIG-T |
| NCBI Synonym Full Names: | phosphatidylinositol glycan anchor biosynthesis class T |
| NCBI Official Symbol: | PIGT |
| NCBI Official Synonym Symbols: | NDAP; PNH2; CGI-06; MCAHS3 |
| NCBI Protein Information: | GPI transamidase component PIG-T |
| UniProt Protein Name: | GPI transamidase component PIG-T |
| UniProt Synonym Protein Names: | Phosphatidylinositol-glycan biosynthesis class T protein |
| Protein Family: | GPI transamidase component |
| UniProt Gene Name: | PIGT |
 | Western blot analysis of extracts of various cell lines, using PIGT antibody (CAB12119) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
