Anti-PICALM Antibody (CAB10575)

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SKU:
CAB10575
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-PICALM Antibody
Antibody SKU:CAB10575
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 290-460 of human PICALM (NP_001008660.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-251MG, HepG2, MCF7, HeLa, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 290-460 of human PICALM (NP_001008660.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IKDS TAAS RATT LSNA VSSL ASTG LSLT KVDE REKQ AALE EEQA RLKA LKEQ RLKE LAKK PHTS LTTA ASPV STSA GGIM TAPA IDIF STPS SSNS TSKL PNDL LDLQ QPTF HPSV HPMS TASQ VAST WGGF TPSP VAQP HPSA GLNV DFES VFGN KSTN VIVD SGGF DEL
Gene ID:8301
Uniprot:Q13492
Cellular Location:Cytoplasmic vesicle, Golgi apparatus, Membrane, Nucleus, clathrin-coated pit, clathrin-coated vesicle
Calculated MW:59kDa/66kDa/68kDa/70kDa
Observed MW:71kDa
Synonyms:PICALM, CALM, CLTH, LAP
Background:This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:PICALM: Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Oncoprotein; Vesicle

Chromosomal Location of Human Ortholog: 11q14

Cellular Component: AP-2 adaptor complex; cell soma; clathrin coat of coated pit; coated pit; membrane; nucleus; perinuclear region of cytoplasm; postsynaptic membrane; presynaptic membrane; vesicle

Molecular Function:clathrin binding; clathrin heavy chain binding; phosphatidylinositol binding; protein binding

Biological Process: cell proliferation; clathrin cage assembly; endosome transport; iron ion homeostasis; negative regulation of receptor-mediated endocytosis; positive regulation of transcription, DNA-dependent; protein complex assembly; receptor internalization; receptor-mediated endocytosis; regulation of endocytosis; regulation of protein localization; synaptic vesicle maturation; vesicle-mediated transport

Disease: Leukemia, Acute Myeloid

NCBI Summary:This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
UniProt Code:Q13492
NCBI GenInfo Identifier:116242714
NCBI Gene ID:8301
NCBI Accession:Q13492.2
UniProt Secondary Accession:Q13492,O60700, Q4LE54, Q6GMQ6, Q86XZ9, B4DTM3, E9PN05 F8VPG7,
UniProt Related Accession:Q13492
Molecular Weight:70,037 Da
NCBI Full Name:Phosphatidylinositol-binding clathrin assembly protein
NCBI Synonym Full Names:phosphatidylinositol binding clathrin assembly protein
NCBI Official Symbol:PICALM  
NCBI Official Synonym Symbols:LAP; CALM; CLTH  
NCBI Protein Information:phosphatidylinositol-binding clathrin assembly protein
UniProt Protein Name:Phosphatidylinositol-binding clathrin assembly protein
UniProt Synonym Protein Names:Clathrin assembly lymphoid myeloid leukemia protein
Protein Family:Phosphatidylinositol-binding clathrin assembly protein
UniProt Gene Name:PICALM  
UniProt Entry Name:PICAL_HUMAN
Anti-PICALM Antibody (CAB10575)Western blot analysis of extracts of various cell lines, using PICALM antibody (CAB10575) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

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