Anti-PI3 Antibody (CAB5441)
- SKU:
- CAB5441
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PI3 Antibody |
| Antibody SKU: | CAB5441 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-117 of human PI3 (NP_002629.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-87MG, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-117 of human PI3 (NP_002629.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AVTG VPVK GQDT VKGR VPFN GQDP VKGQ VSVK GQDK VKAQ EPVK GPVS TKPG SCPI ILIR CAML NPPN RCLK DTDC PGIK KCCE GSCG MACF VPQ |
| Gene ID: | 5266 |
| Uniprot: | P19957 |
| Cellular Location: | Secreted |
| Calculated MW: | 12kDa |
| Observed MW: | 12kDa |
| Synonyms: | PI3, ESI, SKALP, WAP3, WFDC14, cementoin, elafin |
| Background: | This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. |
| UniProt Protein Function: | elafin: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. |
| UniProt Protein Details: | Protein type:Extracellular matrix; Inhibitor; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: proteinaceous extracellular matrix Molecular Function:serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity Biological Process: copulation |
| NCBI Summary: | This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq, Oct 2008] |
| UniProt Code: | P19957 |
| NCBI GenInfo Identifier: | 119262 |
| NCBI Gene ID: | 5266 |
| NCBI Accession: | P19957.3 |
| UniProt Secondary Accession: | P19957,Q6FG74, E1P618, |
| UniProt Related Accession: | P19957 |
| Molecular Weight: | 12,270 Da |
| NCBI Full Name: | Elafin |
| NCBI Synonym Full Names: | peptidase inhibitor 3, skin-derived |
| NCBI Official Symbol: | PI3 |
| NCBI Official Synonym Symbols: | ESI; WAP3; SKALP; WFDC14; cementoin |
| NCBI Protein Information: | elafin; PI-3; trappin-2; pre-elafin; protease inhibitor WAP3; elastase-specific inhibitor; skin-derived antileukoproteinase; WAP four-disulfide core domain 14; WAP four-disulfide core domain protein 14; protease inhibitor 3, skin-derived (SKALP) |
| UniProt Protein Name: | Elafin |
| UniProt Synonym Protein Names: | Elastase-specific inhibitor; ESI; Peptidase inhibitor 3; PI-3; Protease inhibitor WAP3; Skin-derived antileukoproteinase; SKALP; WAP four-disulfide core domain protein 14 |
| Protein Family: | Elafin |
| UniProt Gene Name: | PI3 |
| UniProt Entry Name: | ELAF_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PI3 antibody (CAB5441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human colon using PI3 antibody (CAB5441) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using PI3 antibody (CAB5441) at dilution of 1:100 (40x lens). |
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