Anti-PHKG1 Antibody (CAB15701)
- SKU:
- CAB15701
- Product type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-PHKG1 Antibody |
| Antibody SKU: | CAB15701 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 245-350 of human PHKG1 (NP_006204.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 245-350 of human PHKG1 (NP_006204.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GNYQ FGSP EWDD YSDT VKDL VSRF LVVQ PQNR YTAE EALA HPFF QQYL VEEV RHFS PRGK FKVI ALTV LASV RIYY QYRR VKPV TREI VIRD PYAL RPLR RLID AY |
| Gene ID: | 5260 |
| Uniprot: | Q16816 |
| Cellular Location: | |
| Calculated MW: | 45kDa/48kDa |
| Observed MW: | 55kDa |
| Synonyms: | PHKG1, PHKG |
| Background: | This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | PHKG1: a Ca(2+)/calmodulin-dependent protein kinase of the PHK family. Phosphorylase kinase is a polymer of 16 chains, four each of alpha, beta, gamma, and delta. Alpha and beta are regulatory chains, gamma is the catalytic chain, and delta is calmodulin. |
| UniProt Protein Details: | Protein type:EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; EC 2.7.11.26; EC 2.7.11.19; CAMK group; PHK family Chromosomal Location of Human Ortholog: 7p11.2 Cellular Component: cytosol Biological Process: glycogen catabolic process |
| NCBI Summary: | This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] |
| UniProt Code: | Q16816 |
| NCBI GenInfo Identifier: | 2833281 |
| NCBI Gene ID: | 5260 |
| NCBI Accession: | Q16816.3 |
| UniProt Secondary Accession: | Q16816,Q75LP5, B7Z1D0, F5H2S1, |
| UniProt Related Accession: | Q16816 |
| Molecular Weight: | 48,867 Da |
| NCBI Full Name: | Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform |
| NCBI Synonym Full Names: | phosphorylase kinase catalytic subunit gamma 1 |
| NCBI Official Symbol: | PHKG1 |
| NCBI Official Synonym Symbols: | PHKG |
| NCBI Protein Information: | phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform |
| UniProt Protein Name: | Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform |
| UniProt Synonym Protein Names: | Phosphorylase kinase subunit gamma-1; Serine/threonine-protein kinase PHKG1 (EC:2.7.11.1, EC:2.7.11.26) |
| Protein Family: | Phosphorylase b kinase |
| UniProt Gene Name: | PHKG1 |
| UniProt Entry Name: | PHKG1_HUMAN |
 | Western blot analysis of extracts of mouse kidney, using PHKG1 antibody (CAB15701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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