Anti-PGK2 항체 (CAB12952)

Antibody Name:	Anti-PGK2 Antibody
Antibody SKU:	CAB12952
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 242-360 of human PGK2 (NP_620061.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, Jurkat, HeLa, HepG2, Mouse testis, Mouse brain, Mouse heart, Rat testis, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 242-360 of human PGK2 (NP_620061.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YTFL KVLN NMEI GASL FDEE GAKI VKDI MAKA QKNG VRIT FPVD FVTG DKFD ENAQ VGKA TVAS GISP GWMG LDCG PESN KNHA QVVA QARL IVWN GPLG VFEW DAFA KGTK ALMD EIV
Gene ID:	5232
Uniprot:	P07205
Cellular Location:	Cytoplasm
Calculated MW:	44kDa
Observed MW:	45kDa

Synonyms:

PGK2, HEL-S-272, PGKB, PGKPS, dJ417L20.2

Background:

This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.

UniProt Protein Function:	PGK2: This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010]
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; EC 2.7.2.3 Chromosomal Location of Human Ortholog: 6p12.3 Cellular Component: nucleus Molecular Function:ATP binding; phosphoglycerate kinase activity Biological Process: phosphorylation
NCBI Summary:	This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010]
UniProt Code:	P07205
NCBI GenInfo Identifier:	21264485
NCBI Gene ID:	5232
NCBI Accession:	P07205.3
UniProt Secondary Accession:	P07205,Q9H107, B2R6Y8,
UniProt Related Accession:	P07205
Molecular Weight:	44,796 Da
NCBI Full Name:	Phosphoglycerate kinase 2
NCBI Synonym Full Names:	phosphoglycerate kinase 2
NCBI Official Symbol:	PGK2
NCBI Official Synonym Symbols:	PGKB; PGKPS; HEL-S-272; dJ417L20.2
NCBI Protein Information:	phosphoglycerate kinase 2
UniProt Protein Name:	Phosphoglycerate kinase 2
UniProt Synonym Protein Names:	Phosphoglycerate kinase, testis specific
Protein Family:	Phosphoglycerate kinase
UniProt Gene Name:	PGK2
UniProt Entry Name:	PGK2_HUMAN

	Western blot analysis of extracts of various cell lines, using PGK2 antibody (CAB12952) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human mammary cancer using PGK2 antibody (CAB12952) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat testis using PGK2 antibody (CAB12952) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using PGK2 antibody (CAB12952) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using PGK2 Polyclonal Antibody (CAB12952) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

PGK2 Antibody (PACO11197)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)