Anti-PGC1 alpha Antibody (CAB11971)
- SKU:
- CAB11971
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PGC1 alpha Antibody |
| Antibody SKU: | CAB11971 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human PGC1 alpha (NP_037393.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HepG2, Mouse heart, Rat heart |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human PGC1 alpha (NP_037393.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FGEI EECT VNLR DDGD SYGF ITYR YTCD AFAA LENG YTLR RSNE TDFE LYFC GRKQ FFKS NYAD LDSN SDDF DPAS TKSK YDSL DFDS LLKE AQRS LRR |
| Gene ID: | 10891 |
| Uniprot: | Q9UBK2 |
| Cellular Location: | Cytoplasm, Nucleus, PML body |
| Calculated MW: | 14kDa/30kDa/31kDa/33kDa/77kDa/89kDa/91kDa |
| Observed MW: | 91kDa/110kDa |
| Synonyms: | PPARGC1A, LEM6, PGC-1(alpha), PGC-1alpha, PGC-1v, PGC1, PGC1A, PPARGC1, PPARG coactivator 1 alpha, PGC1 alpha |
| Background: | The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. |
| UniProt Protein Function: | PGC-1 alpha: a transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor genes. Interacts with, and regulates the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). Regulates many genes involved in energy metabolism. Provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. May be involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. Its transcription is repressed by ZNF746 which binds to 'insulin response sequences' in its promoter. Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 4p15.2 Cellular Component: DNA-directed RNA polymerase II, core complex; intracellular membrane-bound organelle; nucleoplasm; nucleus Molecular Function:chromatin DNA binding; DNA binding; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; RNA binding; sequence-specific DNA binding; transcription coactivator activity; transcription factor binding; ubiquitin protein ligase binding Biological Process: brown fat cell differentiation; cellular respiration; circadian regulation of gene expression; circadian rhythm; digestion; mitochondrion organization and biogenesis; mRNA processing; negative regulation of neuron apoptosis; positive regulation of fatty acid oxidation; positive regulation of gluconeogenesis; positive regulation of histone acetylation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein complex assembly; protein stabilization; regulation of circadian rhythm; regulation of transcription, DNA-dependent; response to muscle activity; RNA splicing; thermoregulation; transcription initiation from RNA polymerase II promoter |
| NCBI Summary: | The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UBK2 |
| NCBI GenInfo Identifier: | 47117335 |
| NCBI Gene ID: | 10891 |
| NCBI Accession: | Q9UBK2.1 |
| UniProt Secondary Accession: | Q9UBK2,Q3LIG1, Q4W5M7, Q9UN32, B7Z406, G8DM16, I3RTT5 I3RTT6, I3RTT7, I3RTT8, I3RTT9, |
| UniProt Related Accession: | Q9UBK2 |
| Molecular Weight: | 91kDa |
| NCBI Full Name: | Peroxisome proliferator-activated receptor gamma coactivator 1-alpha |
| NCBI Synonym Full Names: | PPARG coactivator 1 alpha |
| NCBI Official Symbol: | PPARGC1A |
| NCBI Official Synonym Symbols: | LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha) |
| NCBI Protein Information: | peroxisome proliferator-activated receptor gamma coactivator 1-alpha |
| UniProt Protein Name: | Peroxisome proliferator-activated receptor gamma coactivator 1-alpha |
| UniProt Synonym Protein Names: | Ligand effect modulator 6 |
| Protein Family: | Peroxisome proliferator-activated receptor gamma coactivator |
| UniProt Gene Name: | PPARGC1A |
 | Western blot analysis of extracts of various cell lines, using PGC1 alpha antibody (CAB11971) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Western blot analysis of extracts of various cell lines, using PGC1 antibody (CAB11971) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
 | Immunofluorescence analysis of HeLa cells using PGC1 alpha antibody (CAB11971) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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