Anti-PFKFB2 Antibody (CAB9311)
- SKU:
- CAB9311
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PFKFB2 Antibody |
| Antibody SKU: | CAB9311 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 446-505 of human PFKFB2 (NP_006203.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, Mouse liver, Mouse brain, Mouse skeletal muscle, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 446-505 of human PFKFB2 (NP_006203.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RDKP TNNF PKNQ TPVR MRRN SFTP LSSS NTIR RPRN YSVG SRPL KPLS PLRA QDMQ EGAD |
| Gene ID: | 5208 |
| Uniprot: | O60825 |
| Cellular Location: | |
| Calculated MW: | 54kDa/58kDa |
| Observed MW: | 54kDa |
| Synonyms: | PFKFB2, PFK-2/FBPase-2 |
| Background: | The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2, 6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2, 6-bisphosphate, and a fructose-2, 6-biphosphatase activity that catalyzes the degradation of fructose-2, 6-bisphosphate. This protein regulates fructose-2, 6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2, 6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. |
| UniProt Protein Function: | PFKFB2: a bifunctional heterodimeric enzyme involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. Regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. Two splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:EC 3.1.3.46; Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.105; Kinase, other; Phosphatase (non-protein); Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1q31 Cellular Component: cytosol Molecular Function:6-phosphofructo-2-kinase activity; protein binding; protein kinase binding Biological Process: glycolysis |
| NCBI Summary: | The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O60825 |
| NCBI GenInfo Identifier: | 12643333 |
| NCBI Gene ID: | 5208 |
| NCBI Accession: | O60825.2 |
| UniProt Secondary Accession: | O60825,O60824, Q5VVQ3, Q5VVQ4, Q9H3P1, |
| UniProt Related Accession: | O60825 |
| Molecular Weight: | 54,406 Da |
| NCBI Full Name: | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 |
| NCBI Synonym Full Names: | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 |
| NCBI Official Symbol: | PFKFB2 |
| NCBI Official Synonym Symbols: | PFK-2/FBPase-2 |
| NCBI Protein Information: | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 |
| UniProt Protein Name: | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 |
| UniProt Synonym Protein Names: | 6PF-2-K/Fru-2,6-P2ase heart-type isozymeIncluding the following 2 domains:6-phosphofructo-2-kinase (EC:2.7.1.105)Fructose-2,6-bisphosphatase (EC:3.1.3.46) |
| Protein Family: | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase |
| UniProt Gene Name: | PFKFB2 |
| UniProt Entry Name: | F262_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PFKFB2 antibody (CAB9311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of C6 cells using PFKFB2 Rabbit pAb (CAB9311) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using PFKFB2 Rabbit pAb (CAB9311) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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