Anti-PEA15 Antibody (CAB9956)
- SKU:
- CAB9956
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-PEA15 Antibody |
| Antibody SKU: | CAB9956 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PEA15 (NP_003759.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | SH-SY5Y, A375, HeLa, HepG2, NIH/3T3, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PEA15 (NP_003759.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAEY GTLL QDLT NNIT LEDL EQLK SACK EDIP SEKS EEIT TGSA WFSF LESH NKLD KDNL SYIE HIFE ISRR PDLL TMVV DYRT RVLK ISEE DELD TKLT RIPS AKKY KDII RQPS EEEI IKLA PPPK KA |
| Gene ID: | 8682 |
| Uniprot: | Q15121 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 15kDa/17kDa |
| Observed MW: | 15kDa |
| Synonyms: | PEA15, HMAT1, HUMMAT1H, MAT1, MAT1H, PEA-15, PED |
| Background: | This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | PEA-15: an ubiquitously expressed anti-apoptotic protein. Inhibits both Fas- and Tnfrsf1a/Tnfr1-mediated caspase-8 activity and apoptosis. Interacts with CASP8/FLICE and FADD. Regulates glucose transport by controlling both the content of GLUT1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of GLUT4 from the cell interior to the surface. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Apoptosis Chromosomal Location of Human Ortholog: 1q21.1 Cellular Component: cytosol; microtubule associated complex Molecular Function:protein binding Biological Process: activation of MAPK activity; activation of MAPKK activity; apoptosis; axon guidance; carbohydrate transport; DNA damage checkpoint; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; negative regulation of glucose import; nerve growth factor receptor signaling pathway; Ras protein signal transduction; response to morphine; small GTPase mediated signal transduction; transport; vascular endothelial growth factor receptor signaling pathway |
| NCBI Summary: | This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q15121 |
| NCBI GenInfo Identifier: | 32470612 |
| NCBI Gene ID: | 8682 |
| NCBI Accession: | Q15121.2 |
| UniProt Secondary Accession: | Q15121,O00511, B1AKZ3, |
| UniProt Related Accession: | Q15121 |
| Molecular Weight: | 17,307 Da |
| NCBI Full Name: | Astrocytic phosphoprotein PEA-15 |
| NCBI Synonym Full Names: | phosphoprotein enriched in astrocytes 15 |
| NCBI Official Symbol: | PEA15 |
| NCBI Official Synonym Symbols: | PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H |
| NCBI Protein Information: | astrocytic phosphoprotein PEA-15 |
| UniProt Protein Name: | Astrocytic phosphoprotein PEA-15 |
| UniProt Synonym Protein Names: | 15 kDa phosphoprotein enriched in astrocytes; Phosphoprotein enriched in diabetes; PED |
| Protein Family: | Astrocytic phosphoprotein |
| UniProt Gene Name: | PEA15 |
| UniProt Entry Name: | PEA15_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PEA15 antibody (CAB9956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |