Anti-PDHA2 Antibody (CAB9943)
- SKU:
- CAB9943
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-PDHA2 Antibody |
| Antibody SKU: | CAB9943 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 119-388 of human PDHA2 (NP_005381.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NCI-H460, SKOV3, A-549, Mouse testis, Mouse ovary |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 119-388 of human PDHA2 (NP_005381.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HGVC YTRG LSVR SILA ELTG RRGG CAKG KGGS MHMY TKNF YGGN GIVG AQGP LGAG IALA CKYK GNDE ICLT LYGD GAAN QGQI AEAF NMAA LWKL PCVF ICEN NLYG MGTS TERA AASP DYYK RGNF IPGL KVDG MDVL CVRE ATKF AANY CRSG KGPI LMEL QTYR YHGH SMSD PGVS YRTR EEIQ EVRS KRDP IIIL QDRM VNSK LATV EELK EIGA EVRK EIDD AAQF ATTD PEPH LEEL GHHI YSSD SSFE VRGA NPWI KFKS VS |
| Gene ID: | 5161 |
| Uniprot: | P29803 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 42kDa |
| Observed MW: | 43kDa |
| Synonyms: | PDHA2, PDHAL |
| Background: |
| UniProt Protein Function: | PDHA2: a testis-specific mitochondrial matrix enzyme that catalyzes the oxidative decarboxylation of pyruvate, producing acetyl-CoA and CO2. A key enzyme in controlling the balance between lipid and glucose oxidation depending on substrate availability. The pyruvate dehydrogenase (PDH) holoenzyme is a multi-enzyme complex (PDHC) that contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. The activity of PDH is tightly regulated by phosphorylation. The phosphorylation of at least one of three specific serine residues in E1 subunit by PDHK inactivates the PDHC, while dephosphorylation by PDP restores its activity. Expressed in postmeiotic spermatogenic cells. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; EC 1.2.4.1; Mitochondrial; Oxidoreductase Chromosomal Location of Human Ortholog: 4q22.3 Cellular Component: mitochondrion; nucleolus; nucleus Molecular Function:pyruvate dehydrogenase (acetyl-transferring) activity Biological Process: pyruvate metabolic process |
| UniProt Code: | P29803 |
| NCBI GenInfo Identifier: | 266687 |
| NCBI Gene ID: | 5161 |
| NCBI Accession: | P29803.1 |
| UniProt Secondary Accession: | P29803,Q0VDI5, Q4VC02, Q6NXQ1, B2R9Q3, |
| UniProt Related Accession: | P29803 |
| Molecular Weight: | 43kDa |
| NCBI Full Name: | Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial |
| NCBI Synonym Full Names: | pyruvate dehydrogenase (lipoamide) alpha 2 |
| NCBI Official Symbol: | PDHA2 |
| NCBI Official Synonym Symbols: | PDHAL |
| NCBI Protein Information: | pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial |
| UniProt Protein Name: | Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial |
| UniProt Synonym Protein Names: | PDHE1-A type II |
| Protein Family: | Pyruvate dehydrogenase E1 component |
| UniProt Gene Name: | PDHA2 |
 | Western blot analysis of extracts of various cell lines, using PDHA2 antibody (CAB9943) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Immunofluorescence analysis of C6 cells using Anti-PDHA2 Polyclonal Antibody (CAB9943) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using Anti-PDHA2 Polyclonal Antibody (CAB9943) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using Anti-PDHA2 Polyclonal Antibody (CAB9943) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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