Anti-PDHA1 Antibody (CAB1895)
- SKU:
- CAB1895
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-PDHA1 Antibody |
| Antibody SKU: | CAB1895 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-390 of human PDHA1 (NP_000275.1). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:50 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart, Mouse skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-390 of human PDHA1 (NP_000275.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FAND ATFE IKKC DLHR LEEG PPVT TVLT REDG LKYY RMMQ TVRR MELK ADQL YKQK IIRG FCHL CDGQ EACC VGLE AGIN PTDH LITA YRAH GFTF TRGL SVRE ILAE LTGR KGGC AKGK GGSM HMYA KNFY GGNG IVGA QVPL GAGI ALAC KYNG KDEV CLTL YGDG AANQ GQIF EAYN MAAL WKLP CIFI CENN RYGM GTSV ERAA ASTD YYKR GDFI PGLR VDGM DILC VREA TRFA AAYC RSGK GPIL MELQ TYRY HGHS MSDP GVSY RTRE EIQE VRSK SDPI MLLK DRMV NSNL ASVE ELKE IDVE VRKE IEDA AQFA TADP EPPL EELG YHIY SSDP PFEV RGAN QWIK FKSV S |
| Gene ID: | 5160 |
| Uniprot: | P08559 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 40kDa/43kDa/44kDa/47kDa |
| Observed MW: | 45kDa |
| Synonyms: | PDHA1, PDHA, PDHAD, PDHCE1A, PHE1A |
| Background: | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | PDHA1: a mitochondrial matrix enzyme that catalyzes the oxidative decarboxylation of pyruvate, producing acetyl-CoA and CO2. A key enzyme in controlling the balance between lipid and glucose oxidation depending on substrate availability. The pyruvate dehydrogenase (PDH) holoenzyme is a multi-enzyme complex (PDHC) that contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. The activity of PDH is tightly regulated by phosphorylation. The phosphorylation of at least one of three specific serine residues in E1 subunit by PDHK inactivates the PDHC, while dephosphorylation by PDP restores its activity. Sites 1, 2, and 3 of PDHA1 are S293, S300, and S232, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. |
| UniProt Protein Details: | Protein type:EC 1.2.4.1; Mitochondrial; Oxidoreductase; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glycolysis and gluconeogenesis Chromosomal Location of Human Ortholog: Xp22.1 Cellular Component: mitochondrial matrix; mitochondrion; nucleus; pyruvate dehydrogenase complex Molecular Function:pyruvate dehydrogenase activity Biological Process: acetyl-CoA biosynthetic process from pyruvate; glyoxylate metabolic process; pyruvate metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; tricarboxylic acid cycle Disease: Pyruvate Dehydrogenase E1-alpha Deficiency |
| NCBI Summary: | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010] |
| UniProt Code: | P08559 |
| NCBI GenInfo Identifier: | 129063 |
| NCBI Gene ID: | 5160 |
| NCBI Accession: | P08559.3 |
| UniProt Secondary Accession: | P08559,Q53H41, Q5JPT8, Q9NP12, Q9UBJ8, Q9UBU0, Q9UNG4 Q9UNG5, A5YVE9, B2R5P7, B7Z3T7, B7Z3X5, |
| UniProt Related Accession: | P08559 |
| Molecular Weight: | 47,580 Da |
| NCBI Full Name: | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial |
| NCBI Synonym Full Names: | pyruvate dehydrogenase (lipoamide) alpha 1 |
| NCBI Official Symbol: | PDHA1 |
| NCBI Official Synonym Symbols: | PDHA; PDHAD; PHE1A; PDHCE1A |
| NCBI Protein Information: | pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial |
| UniProt Protein Name: | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial |
| UniProt Synonym Protein Names: | PDHE1-A type I |
| Protein Family: | Pyruvate dehydrogenase E1 component |
| UniProt Gene Name: | PDHA1 |
| UniProt Entry Name: | ODPA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PDHA1 antibody (CAB1895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human colon using PDHA1 Rabbit pAb (CAB1895) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using PDHA1 antibody (CAB1895) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using PDHA1 antibody (CAB1895) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of U-87MG cells using 3ug PDHA1 antibody (CAB1895). Western blot was performed from the immunoprecipitate using PDHA1 antibody (CAB1895) at a dilition of 1:1000. |
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