Anti-PDE6D Antibody (CAB14762)
- SKU:
- CAB14762
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-PDE6D Antibody |
| Antibody SKU: | CAB14762 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human PDE6D (NP_002592.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, OVCAR3, Mouse brain, Mouse kidney, Mouse lung, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human PDE6D (NP_002592.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSAK DERA REIL RGFK LNWM NLRD AETG KILW QGTE DLSV PGVE HEAR VPKK ILKC KAVS RELN FSST EQME KFRL EQKV YFKG QCLE EWFF EFGF VIPN STNT WQSL IEAA PESQ MMPA SVLT GNVI IETK FFDD DLLV STSR VRLF YV |
| Gene ID: | 5147 |
| Uniprot: | O43924 |
| Cellular Location: | Cytoplasm, Cytoplasmic vesicle membrane, Peripheral membrane protein, cilium basal body, cytoskeleton, cytosol |
| Calculated MW: | 17kDa |
| Observed MW: | 14-15kDa |
| Synonyms: | PDE6D, JBTS22, PDED |
| Background: | This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. |
| UniProt Protein Function: | PDE6D: Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation. Belongs to the PDE6D/unc-119 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 2q35-q36 Cellular Component: cytoplasmic vesicle; cytosol Molecular Function:GTPase inhibitor activity; protein binding; Rab GTPase binding Disease: Joubert Syndrome 22 |
| NCBI Summary: | This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014] |
| UniProt Code: | O43924 |
| NCBI GenInfo Identifier: | 5921809 |
| NCBI Gene ID: | 5147 |
| NCBI Accession: | O43924.1 |
| UniProt Secondary Accession: | O43924,O43250, |
| UniProt Related Accession: | O43924 |
| Molecular Weight: | 17,420 Da |
| NCBI Full Name: | Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta |
| NCBI Synonym Full Names: | phosphodiesterase 6D |
| NCBI Official Symbol: | PDE6D |
| NCBI Official Synonym Symbols: | PDED; JBTS22 |
| NCBI Protein Information: | retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta |
| UniProt Protein Name: | Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta |
| UniProt Synonym Protein Names: | Protein p17 |
| Protein Family: | Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase |
| UniProt Gene Name: | PDE6D |
| UniProt Entry Name: | PDE6D_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PDE6D antibody (CAB14762) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of L929 cells using PDE6D Polyclonal Antibody (CAB14762) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using PDE6D Polyclonal Antibody (CAB14762) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
