Anti-PDCD6 Antibody (CAB19924)[KO Validated]

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SKU:
CAB19924
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cardiovascular
Frequently bought together:

Description

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Antibody Name:Anti-PDCD6 Antibody [KO Validated]
Antibody SKU:CAB19924
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human PDCD6.
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human PDCD6.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:10016
Uniprot:O75340
Cellular Location:Endoplasmic reticulum membrane, Endosome, Nucleus, Nucleus membrane, Peripheral membrane protein
Calculated MW:7kDa/21kDa
Observed MW:16KDa
Anti-PDCD6 Antibody [KO Validated] (CAB19924)Western blot analysis of extracts from normal (control) and PDCD6 knockout (KO) 293T cells, using PDCD6 antibody (CAB19924) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:PDCD6, ALG-2, ALG2, PEF1B
Background:This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5.
UniProt Protein Function:PDCD6: Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca(2+)- regulated signals along the death pathway. Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101. Interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 5p15.33

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; endoplasmic reticulum; cytoplasm; cytoplasmic vesicle; nucleus; endosome

Molecular Function:protein dimerization activity; protein binding; protein homodimerization activity; protein anchor; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; calcium-dependent protein binding; molecular adaptor activity

Biological Process: caspase activation; intracellular protein transport; negative regulation of TOR signaling pathway; positive regulation of angiogenesis; negative regulation of protein kinase B signaling cascade; negative regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of caspase activity; positive regulation of endothelial cell proliferation; angiogenesis; proteolysis; response to calcium ion

NCBI Summary:This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5. [provided by RefSeq, May 2012]
UniProt Code:O75340
NCBI GenInfo Identifier:12230420
NCBI Gene ID:10016
NCBI Accession:O75340.1
UniProt Secondary Accession:O75340,Q2YDC2, Q5TZS0, B2RD16, E7ESR3,
UniProt Related Accession:O75340
Molecular Weight:191
NCBI Full Name:Programmed cell death protein 6
NCBI Synonym Full Names:programmed cell death 6
NCBI Official Symbol:PDCD6  
NCBI Official Synonym Symbols:ALG2; ALG-2; PEF1B  
NCBI Protein Information:programmed cell death protein 6; apoptosis-linked gene 2 protein; probable calcium-binding protein ALG-2
UniProt Protein Name:Programmed cell death protein 6
UniProt Synonym Protein Names:Apoptosis-linked gene 2 protein; Probable calcium-binding protein ALG-2
Protein Family:Programmed cell death protein
UniProt Gene Name:PDCD6  
UniProt Entry Name:PDCD6_HUMAN

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