Anti-PDCD4 Antibody (CAB2420)

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SKU:
CAB2420
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
Frequently bought together:

Description

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Antibody Name:Anti-PDCD4 Antibody
Antibody SKU:CAB2420
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP FC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human PDCD4
Application:WB IHC IF IP FC
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:20 - 1:50 FC 1:20 - 1:50
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, MCF7
Immunogen:Recombinant protein of human PDCD4
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:27250
Uniprot:Q53EL6
Cellular Location:Cytoplasm, Nucleus
Calculated MW:50kDa/51kDa
Observed MW:57kDa
Synonyms:PDCD4, H731
Background:This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:PDCD4: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.
UniProt Protein Details:

Protein type:Tumor suppressor; Apoptosis

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function:protein binding; RNA binding

Biological Process: apoptosis; cell aging; negative regulation of apoptosis; negative regulation of cell cycle; negative regulation of JNK activity; negative regulation of transcription, DNA-dependent

NCBI Summary:This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
UniProt Code:Q53EL6
NCBI GenInfo Identifier:317373317
NCBI Gene ID:27250
NCBI Accession:Q53EL6.2
UniProt Secondary Accession:Q53EL6,O15501, Q5VZS6, Q6PJI5, Q8TAR5, Q99834, B2RCV4 B5ME91,
UniProt Related Accession:Q53EL6
Molecular Weight:50,576 Da
NCBI Full Name:Programmed cell death protein 4
NCBI Synonym Full Names:programmed cell death 4 (neoplastic transformation inhibitor)
NCBI Official Symbol:PDCD4  
NCBI Official Synonym Symbols:H731  
NCBI Protein Information:programmed cell death protein 4
UniProt Protein Name:Programmed cell death protein 4
UniProt Synonym Protein Names:Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a
Protein Family:Programmed cell death protein
UniProt Gene Name:PDCD4  
UniProt Entry Name:PDCD4_HUMAN
Anti-PDCD4 Antibody (CAB2420)Western blot analysis of extracts of various cell lines, using PDCD4 antibody (CAB2420). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-PDCD4 Antibody (CAB2420)Immunofluorescence analysis of NIH/3T3 cells using PDCD4 antibody (CAB2420) at dilution of 1:100. Blue: DAPI for nuclear staining.

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