Anti-PCK2 Antibody (CAB8446)
- SKU:
- CAB8446
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IP
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-PCK2 Antibody |
| Antibody SKU: | CAB8446 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PCK2 (NP_004554.2). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 IP 1:20 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, LO2, HepG2, Mouse testis, Mouse brain, Mouse kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PCK2 (NP_004554.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAL YRPG LRLN WHGL SPLG WPSC RSIQ TLRV LSGD LGQL PTGI RDFV EHSA RLCQ PEGI HICD GTEA ENTA TLTL LEQQ GLIR KLPK YNNC WLAR TDPK DVAR VESK TVIV TPSQ RDTV QLPP GGAR GQLG NWMS PADF QRAV DERF PGCM QGRT MYVL PFSM GPVG SPLS RIGV QLTD SAYV VASM RIMT RLGT PVLQ ALGD GDFV KCLH SVGQ PLTG QGEP VSQW PCNP EKTL IGHV PDQR EIIS FGSG YGGN SLLG KKCF ALRI ASRL ARDE GWLA |
| Gene ID: | 5106 |
| Uniprot: | Q16822 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 47kDa/70kDa |
| Observed MW: | 71kDa |
| Synonyms: | PCK2, PEPCK, PEPCK-M, PEPCK2 |
| Background: | This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. |
| UniProt Protein Function: | PCK2: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Monomer. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 4.1.1.32; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; Lyase Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:phosphoenolpyruvate carboxykinase (GTP) activity; phosphoenolpyruvate carboxykinase activity; protein binding Biological Process: gluconeogenesis Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Mitochondrial |
| NCBI Summary: | This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014] |
| UniProt Code: | Q16822 |
| NCBI GenInfo Identifier: | 290457671 |
| NCBI Gene ID: | 5106 |
| NCBI Accession: | Q16822.3 |
| UniProt Secondary Accession: | Q16822,O43253, Q86U01, Q9BV62, |
| UniProt Related Accession: | Q16822 |
| Molecular Weight: | 47,564 Da |
| NCBI Full Name: | Phosphoenolpyruvate carboxykinase |
| NCBI Synonym Full Names: | phosphoenolpyruvate carboxykinase 2, mitochondrial |
| NCBI Official Symbol: | PCK2 |
| NCBI Official Synonym Symbols: | PEPCK; PEPCK2; PEPCK-M |
| NCBI Protein Information: | phosphoenolpyruvate carboxykinase [GTP], mitochondrial |
| UniProt Protein Name: | Phosphoenolpyruvate carboxykinase [GTP], mitochondrial |
| Protein Family: | Protein kinase |
| UniProt Gene Name: | PCK2 |
| UniProt Entry Name: | PCKGM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PCK2 antibody (CAB8446) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using PCK2 Rabbit pAb (CAB8446) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using PCK2 Rabbit pAb (CAB8446) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using PCK2 Rabbit pAb (CAB8446) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using PCK2 Rabbit pAb (CAB8446) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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