Anti-PAX5 Antibody (CAB12048)
- SKU:
- CAB12048
- Product type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PAX5 Antibody |
| Antibody SKU: | CAB12048 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PAX5 (NP_057953.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | K-562, Raji, A-549, Rat heart |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PAX5 (NP_057953.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDLE KNYP TPRT SRTG HGGV NQLG GVFV NGRP LPDV VRQR IVEL AHQG VRPC DISR QLRV SHGC VSKI LGRY YETG SIKP GVIG GSKP KVAT PKVV EKIA |
| Gene ID: | 5079 |
| Uniprot: | Q02548 |
| Cellular Location: | Nucleus |
| Calculated MW: | 31kDa/32kDa/34kDa/35kDa/37kDa/38kDa/42kDa |
| Observed MW: | 38kDa |
| Synonyms: | PAX5, ALL3, BSAP |
| Background: | This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene. |
| NCBI Summary: | This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q02548 |
| NCBI GenInfo Identifier: | 417449 |
| NCBI Gene ID: | 5079 |
| NCBI Accession: | Q02548.1 |
| UniProt Secondary Accession: | Q02548,O75933, Q5SFM2, Q6S728, A3QVP6, A3QVP7, A3QVP8 C0KTF6, C0KTF7, C0KTF8, C0KTF9, C0KTG0, |
| UniProt Related Accession: | Q02548 |
| Molecular Weight: | 34,132 Da |
| NCBI Full Name: | Paired box protein Pax-5 |
| NCBI Synonym Full Names: | paired box 5 |
| NCBI Official Symbol: | PAX5 |
| NCBI Official Synonym Symbols: | ALL3; BSAP |
| NCBI Protein Information: | paired box protein Pax-5 |
| UniProt Protein Name: | Paired box protein Pax-5 |
| UniProt Synonym Protein Names: | B-cell-specific transcription factor; BSAP |
| Protein Family: | Paired box protein |
| UniProt Gene Name: | PAX5 |
| UniProt Entry Name: | PAX5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PAX5 antibody (CAB12048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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