Anti-PAWR 항체 (CAB3063)

Antibody Name:	Anti-PAWR Antibody
Antibody SKU:	CAB3063
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human PAWR

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, HT-29, SKOV3, SW480, HepG2

Immunogen:	Recombinant protein of human PAWR
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	5074
Uniprot:	Q96IZ0
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	36kDa
Observed MW:	43kDa

Synonyms:	PAWR, PAR4, Par-4
Background:	The tumor suppressor WT1 represses and activates transcription. The protein encoded by this gene is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells.

UniProt Protein Function:	PAR-4: a proapoptotic protein that drives trafficking and activation of Fas and Fasl to induce prostate cancer cell apoptosis and tumor regression. Capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappaB transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
UniProt Protein Details:	Protein type:Apoptosis Chromosomal Location of Human Ortholog: 12q21 Cellular Component: cytoplasm; plasma membrane; actin filament; nucleus; actin cytoskeleton Molecular Function:protein binding; leucine zipper domain binding; enzyme binding; actin binding; transcription corepressor activity Biological Process: negative regulation of cell proliferation; actin filament bundle formation; negative regulation of T cell proliferation; interleukin-2 biosynthetic process; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; negative regulation of T cell receptor signaling pathway; negative regulation of B cell proliferation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of amyloid precursor protein biosynthetic process
NCBI Summary:	The tumor suppressor WT1 represses and activates transcription. The protein encoded by this gene is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells. [provided by RefSeq, Jul 2008]
UniProt Code:	Q96IZ0
NCBI GenInfo Identifier:	66773935
NCBI Gene ID:	5074
NCBI Accession:	Q96IZ0.1
UniProt Secondary Accession:	Q96IZ0,O75796, Q6FHY9, Q8N700,
UniProt Related Accession:	Q96IZ0
Molecular Weight:	340
NCBI Full Name:	PRKC apoptosis WT1 regulator protein
NCBI Synonym Full Names:	PRKC, apoptosis, WT1, regulator
NCBI Official Symbol:	PAWR
NCBI Official Synonym Symbols:	PAR4; Par-4
NCBI Protein Information:	PRKC apoptosis WT1 regulator protein; WT1-interacting protein; prostate apoptosis response-4; transcriptional repressor PAR4; prostate apoptosis response 4 protein; prostate apoptosis response protein 4; prostate apoptosis response protein PAR-4
UniProt Protein Name:	PRKC apoptosis WT1 regulator protein
UniProt Synonym Protein Names:	Prostate apoptosis response 4 protein; Par-4
Protein Family:	PRKC apoptosis WT1 regulator protein
UniProt Gene Name:	PAWR
UniProt Entry Name:	PAWR_HUMAN

	Western blot analysis of extracts of various cell lines, using PAWR antibody (CAB3063) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat pancreas using PAWR Antibody (CAB3063) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PAWR Antibody (CAB3063) at dilution of 1:200 (40x lens).

Antibodies	ELISA Kits
PAWR Antibody (PACO01296)	Prostate Apoptosis Response protein-4 Colorimetric Cell-Based ELISA Kit
PAWR Antibody (PACO11080)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)