Anti-PAK4 Antibody (CAB11646)
- SKU:
- CAB11646
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-PAK4 Antibody |
| Antibody SKU: | CAB11646 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human PAK4 (NP_001014831.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | LNCaP, Mouse testis, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human PAK4 (NP_001014831.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EPAT TARG GPGK AGSR GRFA GHSE AGGG SGDR RRAG PEKR PKSS REGS GGPQ ESSR DKRP LSGP DVGT PQPA GLAS GAKL AAGR PFNT YPRA DTDH PSRG AQGE PHDV APNG PSAG GLAI PQSS SSSS RPPT RARG APSP GVLG PHAS EPQL APPA CTPA A |
| Gene ID: | 10298 |
| Uniprot: | O96013 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 47kDa/48kDa/54kDa/64kDa |
| Observed MW: | 70KDa |
| Synonyms: | PAK4 |
| Background: | PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| UniProt Protein Function: | PAK4: a protein kinase of the STE20 family. A mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Interacts specifically with the GTP-bound form of Cdc42 and weakly activates the JNK family of MAP kinases. Overexpressed in several cancers. Required for ras-dependent anchorage-independent growth of tumor cell lines. Four alternatively spliced human isoforms have been reported. |
| UniProt Protein Details: | Protein type:Kinase, protein; EC 2.7.11.1; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKB subfamily Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: focal adhesion; Golgi apparatus Molecular Function:protein binding; receptor signaling protein serine/threonine kinase activity Biological Process: apoptosis; cell growth; cell migration; cell motility; cell proliferation; cytoskeleton organization and biogenesis; mitotic cell cycle; signal transduction |
| NCBI Summary: | PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O96013 |
| NCBI GenInfo Identifier: | 12585288 |
| NCBI Gene ID: | 10298 |
| NCBI Accession: | O96013.1 |
| UniProt Secondary Accession: | O96013,Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8, B4DGG6 |
| UniProt Related Accession: | O96013 |
| Molecular Weight: | |
| NCBI Full Name: | Serine/threonine-protein kinase PAK 4 |
| NCBI Synonym Full Names: | p21 (RAC1) activated kinase 4 |
| NCBI Official Symbol: | PAK4 |
| NCBI Protein Information: | serine/threonine-protein kinase PAK 4 |
| UniProt Protein Name: | Serine/threonine-protein kinase PAK 4 |
| UniProt Synonym Protein Names: | p21-activated kinase 4; PAK-4 |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | PAK4 |
| UniProt Entry Name: | PAK4_HUMAN |
 | Western blot analysis of extracts of LNCaP cells, using PAK4 antibody (CAB11646) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Western blot analysis of extracts of various cell lines, using PAK4 antibody (CAB11646) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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