Anti-OPLAH Antibody (CAB12032)
- SKU:
- CAB12032
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-OPLAH Antibody |
| Antibody SKU: | CAB12032 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1119-1288 of human OPLAH (NP_060040.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562, Mouse brain, Mouse kidney, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1119-1288 of human OPLAH (NP_060040.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TVAG GAGA GPSW HGRS GVHS HMTN TRIT DPEI LESR YPVI LRRF ELRR GSGG RGRF RGGD GVTR ELLF REEA LLSV LTER RAFR PYGL HGGE PGAR GLNL LIRK NGRT VNLG GKTS VTVY PGDV FCLH TPGG GGYG DPED PAPP PGSP PQAL AFPE HGSV YEYR RAQE AV |
| Gene ID: | 26873 |
| Uniprot: | O14841 |
| Cellular Location: | |
| Calculated MW: | 137kDa |
| Observed MW: | 134kDa |
| Synonyms: | OPLAH, 5-Opase, OPLA, OPLAHD |
| Background: | The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD). |
| UniProt Protein Function: | OPLAH: Catalyzes the cleavage of 5-oxo-L-proline to form L- glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate. Defects in OPLAH are the cause of 5-oxoprolinase deficiency (OPLAHD). A disorder characterized by calcium oxalate/carbonate urolithiasis, and excessive urinary 5- oxo-L-proline. Affected individuals have recurrent episodes of vomiting, diarrhea, and abdominal pain. Belongs to the oxoprolinase family. |
| UniProt Protein Details: | Protein type:EC 3.5.2.9; Hydrolase; Other Amino Acids Metabolism - glutathione Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: cytosol Molecular Function:5-oxoprolinase (ATP-hydrolyzing) activity Biological Process: glutathione biosynthetic process Disease: 5-oxoprolinase Deficiency |
| NCBI Summary: | The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD). [provided by RefSeq, Jun 2012] |
| UniProt Code: | O14841 |
| NCBI GenInfo Identifier: | 59802978 |
| NCBI Gene ID: | 26873 |
| NCBI Accession: | O14841.3 |
| UniProt Secondary Accession: | O14841,Q75W65, Q9Y4Q0, A5PKY8, |
| UniProt Related Accession: | O14841 |
| Molecular Weight: | 137kDa |
| NCBI Full Name: | 5-oxoprolinase |
| NCBI Synonym Full Names: | 5-oxoprolinase (ATP-hydrolysing) |
| NCBI Official Symbol: | OPLAH |
| NCBI Official Synonym Symbols: | OPLA; OPLAHD; 5-Opase |
| NCBI Protein Information: | 5-oxoprolinase |
| UniProt Protein Name: | 5-oxoprolinase |
| UniProt Synonym Protein Names: | 5-oxo-L-prolinase; 5-OPase; Pyroglutamase |
| UniProt Gene Name: | OPLAH |
 | Western blot analysis of extracts of various cell lines, using OPLAH antibody (CAB12032) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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