Anti-OLA1 Antibody (CAB11671)
- SKU:
- CAB11671
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-OLA1 Antibody |
| Antibody SKU: | CAB11671 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 297-396 of human OLA1 (NP_037473.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, U-87MG, BxPC-3, K-562 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 297-396 of human OLA1 (NP_037473.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KAGF AALQ LEYF FTAG PDEV RAWT IRKG TKAP QAAG KIHT DFEK GFIM AEVM KYED FKEE GSEN AVKA AGKY RQQG RNYI VEDG DIIF FKFN TPQQ PKKK |
| Gene ID: | 29789 |
| Uniprot: | Q9NTK5 |
| Cellular Location: | Cytoplasm, Nucleus, nucleolus |
| Calculated MW: | 27kDa/31kDa/44kDa |
| Observed MW: | 45kDa |
| Synonyms: | OLA1, DOC45, GBP45, GTBP9, GTPBP9, PTD004 |
| Background: |
| UniProt Protein Function: | OLA1: Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP. Belongs to the GTP1/OBG family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.6.3.-; Nucleolus; Hydrolase Chromosomal Location of Human Ortholog: 2q31.1 Cellular Component: centrosome; cytoplasm; membrane Molecular Function:ATP binding; ATPase activity; protein binding Biological Process: ATP metabolic process |
| NCBI Summary: | This gene encodes a member of the GTPase protein family. The encoded protein interacts with breast cancer-associated gene 1 (BRCA1) and BRCA1-associated RING domain protein (BARD1), and is involved in centrosome regulation. Overexpression of this gene has been observed in multiple types of cancer and may be associated with poor survival. Pseudogenes of this gene have been defined on chromosomes 17 and 22. [provided by RefSeq, Jun 2016] |
| UniProt Code: | Q9NTK5 |
| NCBI GenInfo Identifier: | 25453240 |
| NCBI Gene ID: | 29789 |
| NCBI Accession: | Q9NTK5.2 |
| UniProt Secondary Accession: | Q9NTK5,Q5BJD7, Q8NCI8, Q96CU1, Q96SV2, Q9P1D3, Q9UNY9 Q9Y6G4, D7EHM2, |
| UniProt Related Accession: | Q9NTK5 |
| Molecular Weight: | 31,440 Da |
| NCBI Full Name: | Obg-like ATPase 1 |
| NCBI Synonym Full Names: | Obg-like ATPase 1 |
| NCBI Official Symbol: | OLA1 |
| NCBI Official Synonym Symbols: | DOC45; GBP45; GTBP9; GTPBP9; PTD004 |
| NCBI Protein Information: | obg-like ATPase 1 |
| UniProt Protein Name: | Obg-like ATPase 1 |
| UniProt Synonym Protein Names: | DNA damage-regulated overexpressed in cancer 45; DOC45; GTP-binding protein 9 |
| Protein Family: | Obg-like ATPase |
| UniProt Gene Name: | OLA1 |
| UniProt Entry Name: | OLA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using OLA1 antibody (CAB11671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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