Anti-OGT 항체 (CAB1990)

Antibody Name:	Anti-OGT Antibody
Antibody SKU:	CAB1990
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 857-1036 of human OGT (NP_858059.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	293T, SW620, K-562, A-549, HeLa, BT-474, Mouse spleen, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 857-1036 of human OGT (NP_858059.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RVPN SVLW LLRF PAVG EPNI QQYA QNMG LPQN RIIF SPVA PKEE HVRR GQLA DVCL DTPL CNGH TTGM DVLW AGTP MVTM PGET LASR VAAS QLTC LGCL ELIA KNRQ EYED IAVK LGTD LEYL KKVR GKVW KQRI SSPL FNTK QYTM ELER LYLQ MWEH YAAG NKPD HMIK PVEV TESA
Gene ID:	8473
Uniprot:	O15294
Cellular Location:	Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus
Calculated MW:	74kDa/103kDa/115kDa/116kDa
Observed MW:	117kDa

Synonyms:

OGT, HINCUT-1, HRNT1, O-GLCNAC, MRX106, OGT1

Background:

This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

UniProt Protein Function:	Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity (PubMed:22923583). Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3) (PubMed:22121020, PubMed:23353889). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:20018852). O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex (PubMed:24474760). Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2 (PubMed:12150998, PubMed:18288188, PubMed:19377461, PubMed:19451179, PubMed:20018868, PubMed:20200153, PubMed:21285374, PubMed:15361863).
NCBI Summary:	This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Code:	O15294
NCBI GenInfo Identifier:	68067509
NCBI Gene ID:	8473
NCBI Accession:	O15294.3
UniProt Secondary Accession:	O15294,Q7Z3K0, Q8WWM8, Q96CC1, Q9UG57,
UniProt Related Accession:	O15294
Molecular Weight:	74,536 Da
NCBI Full Name:	UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
NCBI Synonym Full Names:	O-linked N-acetylglucosamine (GlcNAc) transferase
NCBI Official Symbol:	OGT
NCBI Official Synonym Symbols:	HRNT1; HINCUT-1; O-GLCNAC
NCBI Protein Information:	UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
UniProt Protein Name:	UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
UniProt Synonym Protein Names:	O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit; OGT
Protein Family:	Methylated-DNA--protein-cysteine methyltransferase
UniProt Gene Name:	OGT
UniProt Entry Name:	OGT1_HUMAN

	Western blot analysis of extracts of various cell lines, using OGT antibody (CAB1990) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of U2OS cells using OGT antibody (CAB1990). Blue: DAPI for nuclear staining.

Antibodies

Anti-OGT Antibody (CAB2214)

Anti-OGT Antibody (CAB3501)

OGT Antibody (PACO11000)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)