Anti-OAZ1 Antibody (CAB7444)
- SKU:
- CAB7444
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-OAZ1 Antibody |
| Antibody SKU: | CAB7444 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-68 of human OAZ1 (NP_004143.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, Mouse heart, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-68 of human OAZ1 (NP_004143.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVKS SLQR ILNS HCFA REKE GDKP SATI HASR TMPL LSLH SRGG SSSE SSRV SLHC CSNP GPGP RWCS |
| Gene ID: | 4946 |
| Uniprot: | P54368 |
| Cellular Location: | |
| Calculated MW: | 25kDa |
| Observed MW: | 27kDa |
| Synonyms: | OAZ1, AZ1, AZI, OAZ |
| Background: | The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 1, the first member of the antizyme family, that has broad tissue distribution, and negatively regulates intracellular polyamine levels by binding to and targeting ODC for degradation, as well as inhibiting polyamine uptake. Antizyme 1 mRNA contains two potential in-frame AUGs; and studies in rat suggest that alternative use of the two translation initiation sites results in N-terminally distinct protein isoforms with different subcellular localization. Alternatively spliced transcript variants have also been noted for this gene. |
| UniProt Protein Function: | OAZ1: Binds to, and destabilizes, ornithine decarboxylase which is then degraded. Also inhibits cellular uptake of polyamines by inactivating the polyamine uptake transporter. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Belongs to the ODC antizyme family. 1 isoforms of the human protein are produced by ribosomal frameshifting. |
| UniProt Protein Details: | Protein type:Inhibitor Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: cytosol Molecular Function:protein binding; ornithine decarboxylase inhibitor activity Biological Process: transport; polyamine biosynthetic process; negative regulation of catalytic activity; positive regulation of protein catabolic process; regulation of amino acid metabolic process |
| NCBI Summary: | The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 1, the first member of the antizyme family, that has broad tissue distribution, and negatively regulates intracellular polyamine levels by binding to and targeting ODC for degradation, as well as inhibiting polyamine uptake. Antizyme 1 mRNA contains two potential in-frame AUGs; and studies in rat suggest that alternative use of the two translation initiation sites results in N-terminally distinct protein isoforms with different subcellular localization. Alternatively spliced transcript variants have also been noted for this gene. [provided by RefSeq, Dec 2014] |
| UniProt Code: | P54368 |
| NCBI GenInfo Identifier: | 1709427 |
| NCBI Gene ID: | 4946 |
| NCBI Accession: | P54368.3 |
| UniProt Secondary Accession: | P54368,O43382, Q14989, Q92595, Q9UPL9, |
| UniProt Related Accession: | P54368 |
| Molecular Weight: | 228 |
| NCBI Full Name: | Ornithine decarboxylase antizyme 1 |
| NCBI Synonym Full Names: | ornithine decarboxylase antizyme 1 |
| NCBI Official Symbol: | OAZ1 |
| NCBI Official Synonym Symbols: | AZI; OAZ |
| NCBI Protein Information: | ornithine decarboxylase antizyme 1; ODC-Az |
| UniProt Protein Name: | Ornithine decarboxylase antizyme 1 |
| Protein Family: | Ornithine decarboxylase antizyme |
| UniProt Gene Name: | OAZ1 |
| UniProt Entry Name: | OAZ1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using OAZ1 antibody (CAB7444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using OAZ1 antibody (CAB7444) at dilution of 1:100 (40x lens). |
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