Anti-OAS3 Antibody (CAB9481)
- SKU:
- CAB9481
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| Antibody Name: | Anti-OAS3 Antibody |
| Antibody SKU: | CAB9481 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 320-620 of human OAS3 (NP_006178.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, DU145, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 320-620 of human OAS3 (NP_006178.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AASC YDHP CFLR GMGD PVQS WKGP GLPR AGCS GLGH PIQL DPNQ KTPE NSKS LNAV YPRA GSKP PSCP APGP TGAA SIVP SVPG MALD LSQI PTKE LDRF IQDH LKPS PQFQ EQVK KAID IILR CLHE NCVH KASR VSKG GSFG RGTD LRDG CDVE LIIF LNCF TDYK DQGP RRAE ILDE MRAQ LESW WQDQ VPSL SLQF PEQN VPEA LQFQ LVST ALKS WTDV SLLP AFDA VGQL SSGT KPNP QVYS RLLT SGCQ EGEH KACF AELR RNFM NIRP VKLK NLIL LVKH WYRQ VAAQ NKGK G |
| Gene ID: | 4940 |
| Uniprot: | Q9Y6K5 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 121kDa |
| Observed MW: | 121kDa |
| Synonyms: | OAS3, p100, p100OAS |
| Background: | This gene encodes an enzyme included in the 2', 5' oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2', 5' oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance. |
| UniProt Protein Function: | OAS3: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes preferentially dimers of 2'-5'- oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. Displays antiviral activity against Chikungunya virus (CHIKV), Dengue virus, Sindbis virus (SINV) and Semliki forest virus (SFV). Belongs to the 2-5A synthase family. |
| UniProt Protein Details: | Protein type:EC 2.7.7.84; Transferase Chromosomal Location of Human Ortholog: 12q24.2 Cellular Component: cytoplasm; cytosol; extracellular space; intracellular membrane-bound organelle; nucleoplasm; plasma membrane Molecular Function:2'-5'-oligoadenylate synthetase activity; ATP binding; double-stranded RNA binding; protein binding Biological Process: defense response to virus; negative regulation of viral genome replication; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; response to virus |
| NCBI Summary: | This gene encodes an enzyme included in the 2', 5' oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2', 5' oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9Y6K5 |
| NCBI GenInfo Identifier: | 317373408 |
| NCBI Gene ID: | 4940 |
| NCBI Accession: | Q9Y6K5.3 |
| UniProt Secondary Accession: | Q9Y6K5,Q2HJ14, Q9H3P5, |
| UniProt Related Accession: | Q9Y6K5 |
| Molecular Weight: | 121,170 Da |
| NCBI Full Name: | 2'-5'-oligoadenylate synthase 3 |
| NCBI Synonym Full Names: | 2'-5'-oligoadenylate synthetase 3 |
| NCBI Official Symbol: | OAS3 |
| NCBI Official Synonym Symbols: | p100; p100OAS |
| NCBI Protein Information: | 2'-5'-oligoadenylate synthase 3 |
| UniProt Protein Name: | 2'-5'-oligoadenylate synthase 3 |
| UniProt Synonym Protein Names: | p100 OAS; p100OAS |
| Protein Family: | 2'-5'-oligoadenylate synthase |
| UniProt Gene Name: | OAS3 |
| UniProt Entry Name: | OAS3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using OAS3 antibody (CAB9481) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of L929 cells using OAS3 antibody (CAB9481) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using OAS3 antibody (CAB9481) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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