Anti-NTAN1 Antibody (CAB15562)
- SKU:
- CAB15562
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NTAN1 Antibody |
| Antibody SKU: | CAB15562 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human NTAN1 (NP_775745.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, Raji, HepG2, MCF-7, Mouse spleen, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human NTAN1 (NP_775745.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPLL VEGR RVRL PQSA GDLV RAHP PLEE RARL LRGQ SVQQ VGPQ GLLY VQQR ELAV TSPK DGSI SILG SDDA TTCH IVVL RHTG NGAT CLTH CDGT DTKA EVPL IMNS IKSF SDHA QCGR LEVH LVGG FSDD RQLS QKLT HQLL SEFD RQED DIHL VTLC VTEL NDRE ENEN HFPV IYGI AVNI KTAE IYRA SFQD RGPE EQLR AART LAGG PMIS IYDA ETEQ LRIG PYSW TPFP HVDF WLHQ DDKQ ILEN LSTS PLAE PPHF VEHI RSTL MFLK KHPS PAHT LFSG NKAL LYKK NEDG LWEK ISSP GS |
| Gene ID: | 123803 |
| Uniprot: | Q96AB6 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 34kDa |
| Observed MW: | 35kDa |
| Synonyms: | NTAN1, PNAA, PNAD |
| Background: | The protein encoded by this gene functions in a step-wise process of protein degradation through the N-end rule pathway. This protein acts as a tertiary destabilizing enzyme that deamidates N-terminal L-Asn residues on proteins to produce N-terminal L-Asp. L-Asp substrates are subsequently conjugated to L-Arg, which is recognized by specific E3 ubiquitin ligases and targeted to the proteasome. Pseudogenes of this gene are located on the long arms of chromosomes 8, 10 and 12. Alternative splicing results in multiple transcript variants that encode different protein isoforms. |
| UniProt Protein Function: | NTAN1: Side-chain deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N- terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position. |
| UniProt Protein Details: | Protein type:Hydrolase; EC 3.5.1.- Chromosomal Location of Human Ortholog: 16p13.11 |
| NCBI Summary: | The protein encoded by this gene functions in a step-wise process of protein degradation through the N-end rule pathway. This protein acts as a tertiary destabilizing enzyme that deamidates N-terminal L-Asn residues on proteins to produce N-terminal L-Asp. L-Asp substrates are subsequently conjugated to L-Arg, which is recognized by specific E3 ubiquitin ligases and targeted to the proteasome. Pseudogenes of this gene are located on the long arms of chromosomes 8, 10 and 12. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012] |
| UniProt Code: | Q96AB6 |
| NCBI GenInfo Identifier: | 37082118 |
| NCBI Gene ID: | 123803 |
| NCBI Accession: | Q96AB6.3 |
| UniProt Secondary Accession: | Q96AB6,Q7Z4Z0, |
| UniProt Related Accession: | Q96AB6 |
| Molecular Weight: | 34,677 Da |
| NCBI Full Name: | Protein N-terminal asparagine amidohydrolase |
| NCBI Synonym Full Names: | N-terminal asparagine amidase |
| NCBI Official Symbol: | NTAN1 |
| NCBI Official Synonym Symbols: | PNAA; PNAD |
| NCBI Protein Information: | protein N-terminal asparagine amidohydrolase |
| UniProt Protein Name: | Protein N-terminal asparagine amidohydrolase |
| UniProt Synonym Protein Names: | Protein NH2-terminal asparagine amidohydrolase; PNAA; Protein NH2-terminal asparagine deamidase; PNAD; Protein N-terminal Asn amidase; Protein N-terminal asparagine amidase; Protein NTN-amidase |
| Protein Family: | Protein N-terminal asparagine amidohydrolase |
| UniProt Gene Name: | NTAN1 |
| UniProt Entry Name: | NTAN1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NTAN1 antibody (CAB15562) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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