Anti-NSUN2 Antibody (CAB3443)
- SKU:
- CAB3443
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-NSUN2 Antibody |
| Antibody SKU: | CAB3443 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 508-767 of human NSUN2 (NP_060225.4). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, Mouse liver, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 508-767 of human NSUN2 (NP_060225.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KKMK LFGF KEDP FVFI PEDD PLFP PIEK FYAL DPSF PRMN LLTR TTEG KKRQ LYMV SKEL RNVL LNNS EKMK VINT GIKV WCRN NSGE EFDC AFRL AQEG IYTL YPFI NSRI ITVS MEDV KILL TQEN PFFR KLSS ETYS QAKD LAKG SIVL KYEP DSAN PDAL QCPI VLCG WRGK ASIR TFVP KNER LHYL RMMG LEVL GEKK KEGV ILTN ESAA STGQ PDND VTEG QRAG EPNS PDAE EANS PDVT AGCD PAGV HPPR |
| Gene ID: | 54888 |
| Uniprot: | Q08J23 |
| Cellular Location: | Cytoplasm, Nucleus, cytoskeleton, nucleolus, spindle |
| Calculated MW: | 59kDa/82kDa/86kDa |
| Observed MW: | 105kDa |
| Synonyms: | NSUN2, MISU, MRT5, SAKI, TRM4 |
| Background: | This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. |
| UniProt Protein Function: | NSUN2: an RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity. Interacts with NPM1 and NCL during interphase. Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA. Defects in NSUN2 are the cause of mental retardation autosomal recessive type 5 (MRT5), a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily. Two human isoforms produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.1.1.203; Methyltransferase; Nucleolus; RNA-binding Chromosomal Location of Human Ortholog: 5p15.31 Cellular Component: cytoplasm; nucleolus; spindle; nucleus Molecular Function:tRNA (cytosine-5-)-methyltransferase activity; tRNA binding Biological Process: mitosis; cell division; tRNA methylation Disease: Mental Retardation, Autosomal Recessive 5 |
| NCBI Summary: | This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011] |
| UniProt Code: | Q08J23 |
| NCBI GenInfo Identifier: | 148887180 |
| NCBI Gene ID: | 54888 |
| NCBI Accession: | Q08J23.2 |
| UniProt Secondary Accession: | Q08J23,Q1HFZ0, |
| UniProt Related Accession: | Q08J23 |
| Molecular Weight: | 767 |
| NCBI Full Name: | tRNA (cytosine(34)-C(5))-methyltransferase |
| NCBI Synonym Full Names: | NOP2/Sun RNA methyltransferase family, member 2 |
| NCBI Official Symbol: | NSUN2 |
| NCBI Official Synonym Symbols: | MISU; MRT5; SAKI; TRM4 |
| NCBI Protein Information: | tRNA (cytosine(34)-C(5))-methyltransferase; tRNA (cytosine(34)-C(5))-methyltransferase; NOP2/Sun domain family, member 2; tRNA methyltransferase 4 homolog; substrate of AIM1/Aurora kinase B; 5-methycytoisine methyltransferase; NOL1/NOP2/Sun domain family |
| UniProt Protein Name: | tRNA (cytosine(34)-C(5))-methyltransferase |
| UniProt Synonym Protein Names: | Myc-induced SUN domain-containing protein; Misu; NOL1/NOP2/Sun domain family member 2; Substrate of AIM1/Aurora kinase B; tRNA (cytosine-5-)-methyltransferase; tRNA methyltransferase 4 homolog; hTrm4 |
| Protein Family: | tRNA |
| UniProt Gene Name: | NSUN2 |
| UniProt Entry Name: | NSUN2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NSUN2 antibody (CAB3443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of C6 cells using NSUN2 antibody (CAB3443) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using NSUN2 antibody (CAB3443) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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