Anti-NPRL2 Antibody (CAB5859)
- SKU:
- CAB5859
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NPRL2 Antibody |
| Antibody SKU: | CAB5859 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NPRL2 (NP_006536.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, U-937, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NPRL2 (NP_006536.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGSG CRIE CIFF SEFH PTLG PKIT YQVP EDFI SREL FDTV QVYI ITKP ELQN KLIT VTAM EKKL IGCP VCIE HKKY SRNA LLFN LGFV CDAQ AKTC ALEP IVKK LAGY LTTL ELES SFVS MEES KQKL VPIM TILL EELN ASGR CTLP IDES NTIH LKVI EQRP DPPV AQEY DVPV FTKD KEDF FNSQ WDLT TQQI LPYI DGFR HIQK ISAE ADVE LNLV |
| Gene ID: | 10641 |
| Uniprot: | Q8WTW4 |
| Cellular Location: | |
| Calculated MW: | 23kDa/43kDa |
| Observed MW: | 40kDa |
| Synonyms: | NPRL2, FFEVF2, NPR2, NPR2L, TUSC4, NPR2-like |
| Background: |
| UniProt Protein Function: | NPRL2: Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at the Tyr-9 Tyr-373 and Tyr-376 residues. May act as a tumor suppressor. Suppresses cell growth and enhanced sensitivity to various anticancer drugs. Belongs to the NPR2 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Tumor suppressor; Inhibitor Chromosomal Location of Human Ortholog: 3p21.3 Molecular Function:protein binding; GTPase activator activity; protein kinase activity Biological Process: negative regulation of kinase activity; protein amino acid phosphorylation; positive regulation of GTPase activity |
| UniProt Code: | Q8WTW4 |
| NCBI GenInfo Identifier: | 47117604 |
| NCBI Gene ID: | 10641 |
| NCBI Accession: | Q8WTW4.2 |
| UniProt Secondary Accession: | Q8WTW4,Q6FGS2, Q9Y249, Q9Y497, A8K831, |
| UniProt Related Accession: | Q8WTW4 |
| Molecular Weight: | Observed MW 40kDaCalculated MW 23kDa/43kDa |
| NCBI Full Name: | Nitrogen permease regulator 2-like protein |
| NCBI Synonym Full Names: | nitrogen permease regulator-like 2 (S. cerevisiae) |
| NCBI Official Symbol: | NPRL2 |
| NCBI Official Synonym Symbols: | NPR2; NPR2L; TUSC4 |
| NCBI Protein Information: | nitrogen permease regulator 2-like protein; G21 protein; 2810446G01Rik; gene 21 protein; NPR2-like protein; tumor suppressor candidate 4; homologous to yeast nitrogen permease (candidate tumor suppressor) |
| UniProt Protein Name: | Nitrogen permease regulator 2-like protein |
| UniProt Synonym Protein Names: | Gene 21 protein; G21 protein; Tumor suppressor candidate 4 |
| Protein Family: | Nitrogen permease regulator 2-like protein |
| UniProt Gene Name: | NPRL2 |
| UniProt Entry Name: | NPRL2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NPRL2 antibody (CAB5859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using NPRL2 antibody (CAB5859). Blue: DAPI for nuclear staining. |
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