Anti-NPLOC4 Antibody (CAB3256)
- SKU:
- CAB3256
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-NPLOC4 Antibody |
| Antibody SKU: | CAB3256 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human NPLOC4 (NP_060391.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:1000 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, A-549, LO2, 293T, Mouse liver, Mouse brain, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human NPLOC4 (NP_060391.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAES IIIR VQSP DGVK RITA TKRE TAAT FLKK VAKE FGFQ NNGF SVYI NRNK TGEI TASS NKSL NLLK IKHG DLLF LFPS SLAG PSSE METS VPPG FKVF GAPN VVED EIDQ YLSK QDGK IYRS RDPQ LCRH GPLG KCVH CVPL EPFD EDYL NHLE PPVK HMSF HAYI RKLT GGAD KGKF VALE NISC KIKS GCEG HLPW PNGI CTKC QPSA ITLN RQKY RHVD NIMF ENHT VADR FLDF WRKT GNQH FGYL YGRY TEHK DIPL GIRA EV |
| Gene ID: | 55666 |
| Uniprot: | Q8TAT6 |
| Cellular Location: | Cytoplasm, Endoplasmic reticulum, Nucleus, cytosol |
| Calculated MW: | 68kDa/69kDa |
| Observed MW: | 70kDa |
| Synonyms: | NPLOC4, NPL4, NPL4 homolog |
| Background: |
| UniProt Protein Function: | NPL4: The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Belongs to the NPL4 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Endoplasmic reticulum Chromosomal Location of Human Ortholog: 17qter Cellular Component: endoplasmic reticulum; nuclear envelope-endoplasmic reticulum network; nucleoplasm; nucleus Molecular Function:protein binding; ubiquitin binding; ubiquitin protein ligase binding Biological Process: ER-associated protein catabolic process; Golgi organization and biogenesis; retrograde protein transport, ER to cytosol; ubiquitin-dependent protein catabolic process |
| UniProt Code: | Q8TAT6 |
| NCBI GenInfo Identifier: | 50428974 |
| NCBI Gene ID: | 55666 |
| NCBI Accession: | Q8TAT6.3 |
| UniProt Secondary Accession: | Q8TAT6,Q8N3J1, Q9H8V2, Q9H964, Q9NWR5, Q9P229, |
| UniProt Related Accession: | Q8TAT6 |
| Molecular Weight: | 69,461 Da |
| NCBI Full Name: | Nuclear protein localization protein 4 homolog |
| NCBI Synonym Full Names: | NPL4 homolog, ubiquitin recognition factor |
| NCBI Official Symbol: | NPLOC4 |
| NCBI Official Synonym Symbols: | NPL4 |
| NCBI Protein Information: | nuclear protein localization protein 4 homolog |
| UniProt Protein Name: | Nuclear protein localization protein 4 homolog |
| Protein Family: | Nuclear protein localization protein |
| UniProt Gene Name: | NPLOC4 |
| UniProt Entry Name: | NPL4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NPLOC4 antibody (CAB3256) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of L929 cells using NPLOC4 Rabbit pAb (CAB3256) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using NPLOC4 Rabbit pAb (CAB3256) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
Anti-WBP1 Antibody (CAB13212)
Anti-STAB2 Antibody (CAB10074)
