Anti-NPL Antibody (CAB9210)
- SKU:
- CAB9210
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-NPL Antibody |
| Antibody SKU: | CAB9210 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NPL (NP_110396.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse eye, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NPL (NP_110396.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAFP KKKL QGLV AATI TPMT ENGE INFS VIGQ YVDY LVKE QGVK NIFV NGTT GEGL SLSV SERR QVAE EWVT KGKD KLDQ VIIH VGAL SLKE SQEL AQHA AEIG ADGI AVIA PFFL KPWT KDIL INFL KEVA AAAP ALPF YYYH IPAL TGVK IRAE ELLD GILD KIPT FQGL KFSD TDLL DFGQ CVDQ NRQQ QFAF LFGV DEQL LSAL VMGA TGAV GSTY NYLG KKTN QMLE AFEQ KDFS LALN YQFC IQ |
| Gene ID: | 80896 |
| Uniprot: | Q9BXD5 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 12kDa/26kDa/31kDa/33kDa/35kDa |
| Observed MW: | 37kDa |
| Synonyms: | NPL, C112, C1orf13, NAL, NPL1 |
| Background: | This gene encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. N-acetylneuraminate lyases regulate cellular concentrations of N-acetyl-neuraminic acid (sialic acid) by mediating the reversible conversion of sialic acid into N-acetylmannosamine and pyruvate. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | NPL: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Belongs to the DHDPS family. NanA subfamily. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 4.1.3.3; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Lyase Chromosomal Location of Human Ortholog: 1q25 Cellular Component: cytosol Molecular Function:N-acetylneuraminate lyase activity; protein binding Biological Process: N-acetylneuraminate catabolic process |
| NCBI Summary: | This gene encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. N-acetylneuraminate lyases regulate cellular concentrations of N-acetyl-neuraminic acid (sialic acid) by mediating the reversible conversion of sialic acid into N-acetylmannosamine and pyruvate. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
| UniProt Code: | Q9BXD5 |
| NCBI GenInfo Identifier: | 74752428 |
| NCBI Gene ID: | 80896 |
| NCBI Accession: | Q9BXD5.1 |
| UniProt Secondary Accession: | Q9BXD5,Q4G0Q8, Q4G0Z2, Q64L88, Q6PEL0, B2R839, |
| UniProt Related Accession: | Q9BXD5 |
| Molecular Weight: | 12,201 Da |
| NCBI Full Name: | N-acetylneuraminate lyase |
| NCBI Synonym Full Names: | N-acetylneuraminate pyruvate lyase |
| NCBI Official Symbol: | NPL |
| NCBI Official Synonym Symbols: | NAL; C112; NPL1; C1orf13 |
| NCBI Protein Information: | N-acetylneuraminate lyase |
| UniProt Protein Name: | N-acetylneuraminate lyase |
| UniProt Synonym Protein Names: | N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialate-pyruvate lyase; Sialic acid aldolase; Sialic acid lyase |
| Protein Family: | NPL4-like protein |
| UniProt Gene Name: | NPL |
| UniProt Entry Name: | NPL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NPL antibody (CAB9210) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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