Anti-NPHS2 Antibody (CAB17337)
- SKU:
- CAB17337
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NPHS2 Antibody |
| Antibody SKU: | CAB17337 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human NPHS2. |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, 293T, mouse testis, mouse kidney, mouse brain, rat spleen |
| Immunogen: | Recombinant protein of human NPHS2. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 7827 |
| Uniprot: | Q9NP85 |
| Cellular Location: | |
| Calculated MW: | 42kDa |
| Observed MW: | 42kDa |
| Synonyms: | NPHS2, PDCN, SRN1, podocin |
| Background: | This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | NPHS2: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2). It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. Belongs to the band 7/mec-2 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 1q25.2 Cellular Component: protein complex; integral to plasma membrane; endoplasmic reticulum; plasma membrane; intercellular junction; lipid raft Molecular Function:protein binding Biological Process: actin cytoskeleton reorganization; excretion Disease: Nephrotic Syndrome, Type 2 |
| NCBI Summary: | This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q9NP85 |
| NCBI GenInfo Identifier: | 12230467 |
| NCBI Gene ID: | 7827 |
| NCBI Accession: | Q9NP85.1 |
| UniProt Secondary Accession: | Q9NP85,Q8N6Q5, B1AM32, B1AM33, |
| UniProt Related Accession: | Q9NP85 |
| Molecular Weight: | 34,421 Da |
| NCBI Full Name: | Podocin |
| NCBI Synonym Full Names: | nephrosis 2, idiopathic, steroid-resistant (podocin) |
| NCBI Official Symbol: | NPHS2 |
| NCBI Official Synonym Symbols: | PDCN; SRN1 |
| NCBI Protein Information: | podocin |
| UniProt Protein Name: | Podocin |
| Protein Family: | Podocin |
| UniProt Gene Name: | NPHS2 |
| UniProt Entry Name: | PODO_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NPHS2 antibody (CAB17337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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