Anti-NOX5 Antibody (CAB7136)
- SKU:
- CAB7136
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cardiovascular
Frequently bought together:
Description
| Antibody Name: | Anti-NOX5 Antibody |
| Antibody SKU: | CAB7136 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 661-765 of human NOX5 (NP_078781.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | 22Rv1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 661-765 of human NOX5 (NP_078781.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AEEA QYGR FLEL HMYM TSAL GKND MKAI GLQM ALDL LANK EKKD SITG LQTR TQPG RPDW SKVF QKVA AEKK GKVQ VFFC GSPA LAKV LKGH CEKF GFRF FQEN F |
| Gene ID: | 79400 |
| Uniprot: | Q96PH1 |
| Cellular Location: | Endoplasmic reticulum, Membrane, Multi-pass membrane protein |
| Calculated MW: | 64kDa/82kDa/83kDa/84kDa/86kDa |
| Observed MW: | 69kDa |
| Synonyms: | NOX5 |
| Background: | This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| UniProt Protein Function: | NOX5: Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Oxidoreductase; EC 1.6.3.-; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 15q23 Cellular Component: endoplasmic reticulum; integral to membrane Molecular Function:protein binding; FAD binding; superoxide-generating NADPH oxidase activity; heme binding; calcium ion binding; hydrogen ion channel activity; NADP binding Biological Process: cell proliferation; proton transport; regulation of fusion of sperm to egg plasma membrane; apoptosis; endothelial cell proliferation; cytokinesis; regulation of proton transport; angiogenesis; cytokine secretion; superoxide release |
| NCBI Summary: | This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | Q96PH1 |
| NCBI GenInfo Identifier: | 74717091 |
| NCBI Gene ID: | 79400 |
| NCBI Accession: | Q96PH1.1 |
| UniProt Secondary Accession: | Q96PH1,Q08AN2, Q08AN3, Q8TEQ1, Q8TER4, Q96PH2, Q96PJ8 Q96PJ9, Q9H6E0, Q9HAM8, B2RBJ4, |
| UniProt Related Accession: | Q96PH1 |
| Molecular Weight: | 83,109 Da |
| NCBI Full Name: | NADPH oxidase 5 |
| NCBI Synonym Full Names: | NADPH oxidase, EF-hand calcium binding domain 5 |
| NCBI Official Symbol: | NOX5 |
| NCBI Protein Information: | NADPH oxidase 5 |
| UniProt Protein Name: | NADPH oxidase 5 |
| Protein Family: | NADPH oxidase |
| UniProt Gene Name: | NOX5 |
| UniProt Entry Name: | NOX5_HUMAN |
 | Western blot analysis of extracts of 22Rv1 cells, using NOX5 antibody (CAB7136) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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