Anti-NOS1 Antibody (CAB1485)
- SKU:
- CAB1485
- Product type:
- Antibody
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NOS1 Antibody |
| Antibody SKU: | CAB1485 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human NOS1 (NP_000611.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Rat |
| Positive Samples: | Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human NOS1 (NP_000611.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEDH MFGV QQIQ PNVI SVRL FKRK VGGL GFLV KERV SKPP VIIS DLIR GGAA EQSG LIQA GDII LAVN GRPL VDLS YDSA LEVL RGIA SETH VVLI LRGP EGFT THLE TTFT GDGT PKTI RVTQ PLGP PTKA VDLS HQPP AGKE QPLA VDGA SGPG NGPQ HAYD DGQE AGSL PHAN GLAP |
| Gene ID: | 4842 |
| Uniprot: | P29475 |
| Cellular Location: | Cell membrane, Cell projection, Peripheral membrane protein, dendritic spine, sarcolemma |
| Calculated MW: | 43kDa/125kDa/148kDa/160kDa/164kDa |
| Observed MW: | 160kDa |
| Synonyms: | NOS1, IHPS1, N-NOS, NC-NOS, NOS, bNOS, nNOS |
| Background: | The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene. |
| UniProt Protein Function: | nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase Chromosomal Location of Human Ortholog: 12q24.22 Cellular Component: cytoplasm; cytoskeleton; cytosol; lipid raft; mitochondrion; nucleoplasm; perinuclear region of cytoplasm; photoreceptor inner segment; plasma membrane; protein complex; sarcolemma; sarcoplasmic reticulum; synapse Molecular Function:cadmium ion binding; FAD binding; FMN binding; heme binding; NADP binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; protein binding; sodium channel regulator activity Biological Process: arginine catabolic process; cell redox homeostasis; exogenous drug catabolic process; multicellular organismal response to stress; myoblast fusion; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of hydrolase activity; negative regulation of potassium ion transport; negative regulation of serotonin uptake; neurotransmitter biosynthetic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; peptidyl-cysteine S-nitrosylation; positive regulation of guanylate cyclase activity; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of sodium ion transport; response to heat; response to hypoxia; striated muscle contraction; vasodilation Disease: Pyloric Stenosis, Infantile Hypertrophic, 1 |
| NCBI Summary: | The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011] |
| UniProt Code: | P29475 |
| NCBI GenInfo Identifier: | 1709333 |
| NCBI Gene ID: | 4842 |
| NCBI Accession: | P29475.2 |
| UniProt Secondary Accession: | P29475,O75713, E9PH30, |
| UniProt Related Accession: | P29475 |
| Molecular Weight: | 161kDa |
| NCBI Full Name: | Nitric oxide synthase, brain |
| NCBI Synonym Full Names: | nitric oxide synthase 1 |
| NCBI Official Symbol: | NOS1 |
| NCBI Official Synonym Symbols: | NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS |
| NCBI Protein Information: | nitric oxide synthase, brain |
| UniProt Protein Name: | Nitric oxide synthase, brain |
| UniProt Synonym Protein Names: | Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS |
| Protein Family: | Nitric oxide synthase |
| UniProt Gene Name: | NOS1 |
 | Western blot analysis of extracts of rat liver, using NOS1 antibody (CAB1485) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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