Anti-NIT1 Antibody (CAB16407)
- SKU:
- CAB16407
- Product type:
- Antibody
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NIT1 Antibody |
| Antibody SKU: | CAB16407 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NIT1 (NP_001172021.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Rat |
| Positive Samples: | Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NIT1 (NP_001172021.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLGF ITRP PHRF LSLL CPGL RIPQ LSVL CAQP RPRA MAIS SSSC ELPL VAVC QVTS TPDK QQNF KTCA ELVR EAAR LGAC LAFL PEAF DFIA RDPA ETLH LSEP LGGK LLEE YTQL AREC GLWL SLGG FHER GQDW EQTQ KIYN CHVL LN |
| Gene ID: | 4817 |
| Uniprot: | Q86X76 |
| Cellular Location: | Cytoplasm, Mitochondrion |
| Calculated MW: | 26kDa/31kDa/34kDa/35kDa/40kDa |
| Observed MW: | 34kDa |
| Synonyms: | NIT1 |
| Background: | This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | NIT1: Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2. Belongs to the UPF0012 family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.5.-.-; Hydrolase Chromosomal Location of Human Ortholog: 1q21-q22 Cellular Component: nucleus Molecular Function:nitrilase activity |
| NCBI Summary: | This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| UniProt Code: | Q86X76 |
| NCBI GenInfo Identifier: | 51704324 |
| NCBI Gene ID: | 4817 |
| NCBI Accession: | Q86X76.2 |
| UniProt Secondary Accession: | Q86X76,O76091, B1AQP3, D3DVF4, |
| UniProt Related Accession: | Q86X76 |
| Molecular Weight: | 26,326 Da |
| NCBI Full Name: | Nitrilase homolog 1 |
| NCBI Synonym Full Names: | nitrilase 1 |
| NCBI Official Symbol: | NIT1 |
| NCBI Protein Information: | nitrilase homolog 1 |
| UniProt Protein Name: | Nitrilase homolog 1 |
| Protein Family: | Nitrilase |
| UniProt Gene Name: | NIT1 |
| UniProt Entry Name: | NIT1_HUMAN |
 | Western blot analysis of extracts of rat liver, using NIT1 antibody (CAB16407) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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