Anti-NHP2 항체 (CAB5991)

Antibody Name:	Anti-NHP2 Antibody
Antibody SKU:	CAB5991
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human NHP2 (NP_060308.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	293T, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human NHP2 (NP_060308.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTKI KADP DGPE AQAE ACSG ERTY QELL VNQN PIAQ PLAS RRLT RKLY KCIK KAVK QKQI RRGV KEVQ KFVN KGEK GIMV LAGD TLPI EVYC HLPV MCED RNLP YVYI PSKT DLGA AAGS KRPT CVIM VKPH EEYQ EAYD ECLE EVQS LPLP L
Gene ID:	55651
Uniprot:	Q9NX24
Cellular Location:	Cajal body, Nucleus, nucleolus
Calculated MW:	17kDa
Observed MW:	17kDa

Synonyms:

NHP2, DKCB2, NHP2P, NOLA2

Background:

This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nhp2p. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	NOLA2: Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine (psi) residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Defects in NHP2 are the cause of dyskeratosis congenita autosomal recessive type 2 (DKCB2). A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy. Belongs to the ribosomal protein L7Ae family.
UniProt Protein Details:	Protein type:Nucleolus Chromosomal Location of Human Ortholog: 5q35.3 Cellular Component: nucleoplasm; Cajal body; small nucleolar ribonucleoprotein complex; cytoplasm; nucleolus Molecular Function:snoRNA binding Biological Process: rRNA pseudouridine synthesis Disease: Dyskeratosis Congenita, Autosomal Recessive, 2; Dyskeratosis Congenita, Autosomal Recessive, 1
NCBI Summary:	This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nhp2p. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]
UniProt Code:	Q9NX24
NCBI GenInfo Identifier:	68565945
NCBI Gene ID:	55651
NCBI Accession:	Q9NX24.1
UniProt Secondary Accession:	Q9NX24,Q9P095, A6NKY8,
UniProt Related Accession:	Q9NX24
Molecular Weight:	153
NCBI Full Name:	H/ACA ribonucleoprotein complex subunit 2
NCBI Synonym Full Names:	NHP2 ribonucleoprotein
NCBI Official Symbol:	NHP2
NCBI Official Synonym Symbols:	DKCB2; NHP2P; NOLA2
NCBI Protein Information:	H/ACA ribonucleoprotein complex subunit 2; NHP2-like protein; snoRNP protein NHP2; NHP2 ribonucleoprotein homolog; nucleolar protein family A, member 2 (H/ACA small nucleolar RNPs)
UniProt Protein Name:	H/ACA ribonucleoprotein complex subunit 2
UniProt Synonym Protein Names:	Nucleolar protein family A member 2; snoRNP protein NHP2
Protein Family:	NHP2-like protein
UniProt Gene Name:	NHP2
UniProt Entry Name:	NHP2_HUMAN

Western blot analysis of extracts of various cell lines, using NHP2 Antibody (CAB5991) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.

Antibodies

NHP2 Antibody (PACO10850)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)