Anti-NGLY1 Antibody (CAB15864)
- SKU:
- CAB15864
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NGLY1 Antibody |
| Antibody SKU: | CAB15864 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NGLY1 (NP_060767.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Raji, Mouse brain, Mouse liver, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NGLY1 (NP_060767.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAA ALGS SSGS ASPA VAEL CQNT PETF LEAS KLLL TYAD NILR NPND EKYR SIRI GNTA FSTR LLPV RGAV ECLF EMGF EEGE THLI FPKK ASVE QLQK IRDL IAIE RSSR LDGS NKSH KVKS SQQP AAST QLPT TPSS NPSG LNQH TRNR QGQS SDPP SAST VAAD SAIL EVLQ SNIQ HVLV YENP ALQE KALA CIPV QELK RKSQ EKLS RARK LDKG INIS DEDF LLLE LLHW FKEE FFHW VNNV LCSK CGGQ TRSR DRSL LPSD DELK WGAK EVED HYCD ACQF SNRF PRYN NPEK |
| Gene ID: | 55768 |
| Uniprot: | Q96IV0 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 14kDa/63kDa/70kDa/72kDa/74kDa |
| Observed MW: | 74kDa |
| Synonyms: | NGLY1, CDDG, CDG1V, PNG1, PNGase |
| Background: | This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. |
| NCBI Summary: | This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009] |
| UniProt Code: | Q96IV0 |
| NCBI GenInfo Identifier: | 13938210 |
| NCBI Gene ID: | 55768 |
| NCBI Accession: | BC007226 |
| UniProt Secondary Accession: | Q96IV0,Q59FB1, Q6PJD8, Q9BVR8, Q9NR70, B4DJE9, |
| UniProt Related Accession: | Q96IV0 |
| Molecular Weight: | 70,211 Da |
| NCBI Full Name: | Homo sapiens N-glycanase 1, mRNA |
| NCBI Synonym Full Names: | N-glycanase 1 |
| NCBI Official Symbol: | NGLY1 |
| NCBI Official Synonym Symbols: | CDDG; PNG1; CDG1V; PNGase |
| NCBI Protein Information: | peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase |
| UniProt Protein Name: | Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase |
| UniProt Synonym Protein Names: | N-glycanase 1; Peptide:N-glycanase |
| UniProt Gene Name: | NGLY1 |
 | Western blot analysis of extracts of various cell lines, using NGLY1 antibody (CAB15864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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