Anti-NGEF Antibody (CAB16507)
- SKU:
- CAB16507
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NGEF Antibody |
| Antibody SKU: | CAB16507 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 429-710 of human NGEF (NP_062824.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | PC-3, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 429-710 of human NGEF (NP_062824.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RVEE RSER ECTA LDAH KELE MVVK ACNE GVRK MSRT EQMI SIQK KMEF KIKS VPII SHSR WLLK QGEL QQMS GPKT SRTL RTKK LFHE IYLF LFND LLVI CRQI PGDK YQVF DSAP RGLL RVEE LEDQ GQTL ANVF ILRL LENA DDRE ATYM LKAS SQSE MKRW MTSL APNR RTKF VSFT SRLL DCPQ VQCV HPYV AQQP DELT LELA DILN ILDK TDDG WIFG ERLH DQER GWFP SSMT EEIL NPKI RSQN LKEC FRVH KMDD PQRS QNKD RRKL GSRN RQ |
| Gene ID: | 25791 |
| Uniprot: | Q8N5V2 |
| Cellular Location: | Cell projection, Cytoplasm, Membrane, growth cone |
| Calculated MW: | 26kDa/71kDa/82kDa |
| Observed MW: | 82kDa |
| Synonyms: | NGEF, ARHGEF27, EPHEXIN, ephexin-1 |
| Background: |
| UniProt Protein Function: | ephexin-1: Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension. Interacts with CDK5R1 and EPHA4; activated by EPHA4 through the CDK5 kinase. Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GEFs, Rac/Rho; GEFs Chromosomal Location of Human Ortholog: 2q37 Cellular Component: cytosol Molecular Function:guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity Biological Process: ephrin receptor signaling pathway; positive regulation of apoptosis; regulation of GTPase activity; regulation of small GTPase mediated signal transduction |
| UniProt Code: | Q8N5V2 |
| NCBI GenInfo Identifier: | 114152090 |
| NCBI Gene ID: | 25791 |
| NCBI Accession: | Q8N5V2.2 |
| UniProt Secondary Accession: | Q8N5V2,Q53QQ4, Q53ST7, Q6GMQ5, Q9NQD6, B4DMB8, B9A045 E9PC42, |
| UniProt Related Accession: | Q8N5V2 |
| Molecular Weight: | 71,387 Da |
| NCBI Full Name: | Ephexin-1 |
| NCBI Synonym Full Names: | neuronal guanine nucleotide exchange factor |
| NCBI Official Symbol: | NGEF |
| NCBI Official Synonym Symbols: | EPHEXIN; ARHGEF27 |
| NCBI Protein Information: | ephexin-1 |
| UniProt Protein Name: | Ephexin-1 |
| UniProt Synonym Protein Names: | Eph-interacting exchange protein; Neuronal guanine nucleotide exchange factor |
| Protein Family: | Ephexin |
| UniProt Gene Name: | NGEF |
| UniProt Entry Name: | NGEF_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NGEF antibody (CAB16507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
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